Anticorps Polyclonal de lapin anti-DR5-Specific

DR5-Specific Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 15497-1-AP

Synonymes

CD262, Death receptor 5, DR5, KILLER, KILLER/DR5, TNFRSF10B, TRAIL R2, TRAIL receptor 2, TRAILR2, TRICK2, TRICK2A, TRICK2B, TRICKB, ZTNFR9

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Galerie de données de validation

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  WB analysis of HL-60 using 15497-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 15497-1-AP (TNFRSF10B-Specific antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 cells using 15497-1-AP

  WB result of 15497-1-AP (TNFRSF10B, DR5 antibody) with PC-3 cell, untreated or treated with tunicamysin.

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 11 publications below

Informations sur le produit

15497-1-AP cible DR5-Specific dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: tumor necrosis factor receptor superfamily, member 10b
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 58-60 kDa, 45-50 kDa, 32 kDa
• Numéro d’acquisition GenBank: NM_003842
• Symbole du gène: DR5
• Identification du gène (NCBI): 8795
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TNFRSF10B, also known as CD262, DR5, TRAILR2, TRICK2 and KILLER, is a widely expressed single-pass type I membrane protein belonging to the tumour necrosis factor receptor superfamily (TNFRSF). It is a receptor for TNF-related apoptosis-inducing ligand (TRAIL), which is a member of the tumor necrosis factor (TNF) family of cytokines and induces apoptosis in a wide variety of cells (PMID: 9311998). TNFRSF10B contains two extracellular cysteine-rich repeats, typical for TNF receptor (TNFR) family members, and a cytoplasmic death domain (DD), through which TNFRSF10B is capable to transmit the apoptotic signal (PMID: 9311998; 20531300). In PDAC cells, especially Panc89 cells, it expresses additionally to the approximately 48 and 40 kDa forms of DR5, a faster migrating variant of DR5 of about 32 kDa(PMID:20354842). The TRAIL receptor 2 (death receptor 5, or DR5) forms receptor dimers in a ligand-dependent manner, and these receptor dimers exist within high molecular weight networks(Dr. Jonathan N. Sachs,2012).

Publications

Species	Application	Title
human	WB	Nat Commun; A non-canonical pathway regulates ER stress signaling and blocks ER stress-induced apoptosis and heart failure.; Authors - Yufeng Yao
human	WB	Int J Biol Sci; ESRG is critical to maintain the cell survival and self-renewal/pluripotency of hPSCs by collaborating with MCM2 to suppress p53 pathway; Authors - Shasha Li
human	WB	Biomed Pharmacother; Pro-survival effects by NF-κB, Akt and ERK(1/2) and anti-apoptosis actions by Six1 disrupt apoptotic functions of TRAIL-Dr4/5 pathway in ovarian cancer.; Authors - Juan Yang
human	WB	iScience; A small-molecule Skp1 inhibitor elicits cell death by p53-dependent mechanism.; Authors - Muzammal Hussain
human	WB	Mol Pharm; Discovery and characterization of 4-hydroxy-2-pyridone derivative sambutoxin as a potent and promising anticancer drug candidate: activity and molecular mechanism.; Authors - Lu-Ning Li
human	WB	Arch Biochem Biophys; Luteolin enhances TRAIL sensitivity in non-small cell lung cancer cells through increasing DR5 expression and Drp1-mediated mitochondrial fission.; Authors - Bin Wu

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