Anticorps Polyclonal de lapin anti-TFEC

TFEC Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Avis (1)

N° de cat : 13547-1-AP

Synonymes

bHLHe34, hTFEC L, TCFEC, TFE C, TFEC, TFECL, transcription factor EC, Transcription factor EC like

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Galerie de données de validation

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  WB analysis of mouse testis using 13547-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13547-1-AP (TFEC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 13547-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 13547-1-AP (TFEC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 13547-1-AP

  IP Result of anti-TFEC (IP:13547-1-AP, 4ug; Detection:13547-1-AP 1:500) with mouse testis tissue lysate 4000ug.

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, cellules COLO 320
• Résultats positifs en IP: tissu testiculaire de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

13547-1-AP cible TFEC dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TFEC Protéine recombinante Ag4061
• Nom complet: transcription factor EC
• Masse moléculaire calculée: 347 aa, 39 kDa
• Poids moléculaire observé: 35-40 kDa, 65 kDa
• Numéro d’acquisition GenBank: BC029891
• Symbole du gène: TFEC
• Identification du gène (NCBI): 22797
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The DNA-binding factor TFE3 contains adjacent helix-loop-helix (HLH) and leucine zipper (LZ) domains flanked by an upstream basic region. These protein motifs are frequently observed in other transcription factors and are particularly common to members of the Myc family. TFEC shares a bHLH/LZ structure with TFE3 and a closely related protein microphthalmia-associated transcription factor (MITF), which is critically involved in melanocyte differentiation. The expression of TFEC is mainly in fibroblasts, myoblasts, chondrosarcoma cells and myeloma cells. 50kDa TFEC isoforms are produced by alternative splicing (PMID: 29303964, PMID: 11467950).

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Weihao (Verified Customer) (02-03-2022)

Works great for mouse BMDM and human MDM, strong signal.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500