Anticorps Polyclonal de lapin anti-TAF12

• Phare
• Validé par KD/KO

TAF12 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 12353-1-AP

Synonymes

TAF12, TAF15, TAF2J, TAFII 20/TAFII 15, TAFII20, TAFII20/TAFII15

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Galerie de données de validation

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  WB analysis of Y79 using 12353-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12353-1-AP (TAF12 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of Y79 using 12353-1-AP

  IP result of anti-TAF12 (IP:12353-1-AP, 4ug; Detection:12353-1-AP 1:300) with Y79 cells lysate 2000ug.

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Applications testées

• Résultats positifs en WB: cellules Y79
• Résultats positifs en IP: cellules Y79

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

12353-1-AP cible TAF12 dans les applications de WB, IHC, IP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TAF12 Protéine recombinante Ag3017
• Nom complet: TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 20kDa
• Masse moléculaire calculée: 18 kDa
• Poids moléculaire observé: 21 kDa
• Numéro d’acquisition GenBank: BC011986
• Symbole du gène: TAF12
• Identification du gène (NCBI): 6883
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TAF12, also named TAF15 or TAF2J, is a TBP-associated factor that is contained in Pol I- and Pol II-specific TBP-TAF complexes, it is also a component of the transcription factor IID (TFIID) complex, which is essential for mediating regulation of RNA polymerase transcription. TAF12 plays a key role in regulating eukaryotic gene expression by directly binding promoters and enhancer-bound transactivator proteins. Likewise, TAF12 recruits Gadd45a and the nucleotide excision repair complex to the promoter of rRNA genes leading to active DNA demethylation. Observed MW of TAF12 is from 14-17 kDa and 17-21 kDa.

Publications

Species	Application	Title
mouse	WB	Cancer Cell; A TFIID-SAGA Perturbation that Targets MYB and Suppresses Acute Myeloid Leukemia.; Authors - Yali Xu ; KD Validated
mouse	WB	Cell Metab; Contributions of the measles virus nucleocapsid gene and the SQSTM1/p62(P392L) mutation to Paget's disease.; Authors - Noriyoshi Kurihara
mouse	ChIP,WB	J Bone Miner Res; Role of ATF7-TAF12 interactions in the vitamin D response hypersensitivity of osteoclast precursors in Paget's disease.; Authors - Jumpei Teramachi
mouse, human	WB	J Bone Miner Res; TBK1 mediates critical effects of measles virus nucleocapsid protein (MVNP) on pagetic osteoclast formation.; Authors - Sun Quanhong Q
mouse	WB	J Bone Miner Res; Increased IL-6 expression in osteoclasts is necessary but not sufficient for the development of Paget's disease of bone.; Authors - Jumpei Teramachi
human	WB	Mol Cell Biol; The general transcription factor TAF7 is essential for embryonic development but not essential for the survival or differentiation of mature T cells.; Authors - Gegonne Anne A

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