Anticorps Polyclonal de lapin anti-SCP3

SCP3 Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

22

Avis (2)

N° de cat : 23024-1-AP

Synonymes

COR1, SCP 3, SCP3, SYCP3, synaptonemal complex protein 3

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Galerie de données de validation

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  WB analysis using 23024-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23024-1-AP (SCP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 23024-1-AP

  Rat testis tissue were subjected to SDS PAGE followed by western blot with 23024-1-AP (SCP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 23024-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23024-1-AP (SCP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 23024-1-AP

  IP result of anti-SCP3 (IP:23024-1-AP, 3ug; Detection:23024-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

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  IF Staining of HepG2 using 23024-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 23024-1-AP (SCP3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, cellules HepG2, tissu testiculaire de rat
• Résultats positifs en IP: tissu testiculaire de souris
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 15 publications below

Informations sur le produit

23024-1-AP cible SCP3 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SCP3 Protéine recombinante Ag19330
• Nom complet: synaptonemal complex protein 3
• Masse moléculaire calculée: 236 aa, 28 kDa
• Poids moléculaire observé: 30 kDa, 33 kDa
• Numéro d’acquisition GenBank: BC062662
• Symbole du gène: SYCP3
• Identification du gène (NCBI): 50511
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SYCP3 is an essential structural component of the synaptonemal complex. This complex is involved in synapsis, recombination and segregation of meiotic chromosomes. SYCP3 is required for centromere pairing during meiosis in male germ cells. SYCP3 is also required for normal meiosis during spermatogenesis and male fertility. Mutations in SYCP3 are associated with azoospermia in males and susceptibility to pregnancy loss in females.

Publications

Species	Application	Title
mouse	WB,IF	Nucleic Acids Res; SCF ubiquitin E3 ligase regulates DNA double-strand breaks in early meiotic recombination.; Authors - Yongjuan Guan
mouse	IHC	Genes Dev; Control of homologous recombination by the HROB-MCM8-MCM9 pathway.; Authors - Nicole Hustedt
mouse	IF	Cell Death Differ; NBS1 is required for SPO11-linked DNA double-strand break repair in male meiosis.; Authors - Bin Zhang
mouse	WB,IF	Int J Nanomedicine; Pseudoephedrine Nanoparticles Alleviate Adriamycin-Induced Reproductive Toxicity Through the GnRhR Signaling Pathway.; Authors - Yang Fu
mouse,rat	WB	Biomed Pharmacother; Ephedra herb reduces adriamycin-induced testicular toxicity by upregulating the gonadotropin-releasing hormone signalling pathway.; Authors - Yang Fu
mouse	IF	Andrology; Exposure to elevated temperature affects the expression of piwi-interacting RNAs and associated transcripts in mouse testes; Authors - Wenbin Chen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Feng-Qian (Verified Customer) (04-03-2020)

It nicely detects meiotic chromosomes on methanol-fixed fresh frozen mouse testis sections.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: mouse testis

FH

Huizhong (Verified Customer) (08-31-2018)

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: mouse testis cells

SCP3 Antibody Immunofluorescence, validation (1:50 dilution) in mouse testis cells (Cat no:23024-1-AP)