Anticorps Polyclonal de lapin anti-STRAP

• Phare
• Validé par KD/KO

STRAP Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 18277-1-AP

Synonymes

MAP activator with WD repeats, MAWD, PT WD, STRAP, UNR interacting protein, UNRIP, WD 40 repeat protein PT WD

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Galerie de données de validation

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  WB analysis using 18277-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18277-1-AP (STRAP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 18277-1-AP

  WB result of STRAP antibody (18277-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STRAP transfected HEK-293 cells.

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  IP experiment of HeLa using 18277-1-AP

  IP result of anti-STRAP (IP:18277-1-AP, 4ug; Detection:18277-1-AP 1:2000) with HeLa cells lysate 2000ug.

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  IHC staining of human ovary tumor using 18277-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 18277-1-AP (STRAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 18277-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 18277-1-AP (STRAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 18277-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18277-1-AP (STRAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 18277-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18277-1-AP (STRAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 18277-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using STRAP antibody (18277-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Informations sur le produit

18277-1-AP cible STRAP dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: STRAP Protéine recombinante Ag13085
• Nom complet: serine/threonine kinase receptor associated protein
• Masse moléculaire calculée: 38 kDa
• Poids moléculaire observé: 39 kDa
• Numéro d’acquisition GenBank: BC000162
• Symbole du gène: STRAP
• Identification du gène (NCBI): 11171
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

STRAP (Serine-threonine kinase receptor-associated protein), also known as MAWD, is a ubiquitous WD40 domain protein, common function of which is to provide a suitable scaffold for coordinating multiprotein complex assemblies and thus, regulate a variety of cellular processes like signal transduction, transcriptional regulation, programmed cell death and so on. STRAP plays a role in the cellular distribution of the SMN complex, which is essential for spliceosomal snRNP assembly in the cytoplasm. STRAP inhibits transforming growth factor-beta (TGF-beta) signaling and enhances tumorigenicity via TGF-beta-dependent and -independent mechanisms. STRAP is up-regulated in several cancers and functions as an oncogene. STRAP imparts oncogenic characteristics to cells by promoting ERK and pRb phosphorylation. Moreover, STRAP regulates c-Jun stability by decreasing the ubiquitylation and proteosomal degradation of c-Jun.

Publications

Species	Application	Title
human	WB,IP	Nucleic Acids Res; Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR.; Authors - Evan P Booy ; KD Validated
human	WB,IP	EMBO J; The p53-inducible long noncoding RNA TRINGS protects cancer cells from necrosis under glucose starvation.; Authors - Muhammad Riaz Khan ; KD Validated
human		Cancers (Basel); Serine-Threonine Kinase Receptor-Associated Protein (STRAP) Knockout Decreases the Malignant Phenotype in Neuroblastoma Cell Lines.; Authors - Laura V Bownes ; KD Validated
human	WB	Front Oncol; STRAP as a New Therapeutic Target for Poor Prognosis of Pancreatic Ductal Adenocarcinoma Patients Mainly Caused by TP53 Mutation.; Authors - Shanshan Hu ; KD Validated
human	WB	J Biol Chem; The noncoding RNA BC200 associates with polysomes to positively regulate mRNA translation in tumor cells.; Authors - Evan P Booy
human	WB,IF	Int J Mol Sci; SIRT7 Deacetylates STRAP to Regulate p53 Activity and Stability.; Authors - Miao Yu

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