SMAD7 Polyclonal antibody

• Featured Product
• KD/KO Validated

SMAD7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

101

Cat no : 25840-1-AP

Synonyms

MADH8, MADH7, MAD homolog 8, MAD homolog 7, hSMAD7

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Validation Data Gallery

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  WB analysis of HepG2 using 25840-1-AP

  WB result of SMAD7 antibody (25840-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMAD7 transfected HepG2 cells.

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  WB analysis using 25840-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25840-1-AP (SMAD7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 25840-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25840-1-AP (SMAD7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 25840-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 25840-1-AP (SMAD7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 25840-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 25840-1-AP (SMAD7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells, NIH/3T3 cells, MCF-7cells, SH-SY5Y cells, SW480 cells
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 6 publications below
• WB: See 98 publications below
• IHC: See 7 publications below
• IF: See 7 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

25840-1-AP targets SMAD7 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SMAD7 fusion protein Ag13688
• Full Name: SMAD family member 7
• Calculated Molecular Weight: 426 aa, 46 kDa
• Observed Molecular Weight: 45-55 kDa
• GenBank Accession Number: BC074819
• Gene Symbol: SMAD7
• Gene ID (NCBI): 4092
• RRID: AB_2848137
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMAD7, also named as Mothers against decapentaplegic homolog 7, is a 426 amino acid protein, which belongs to the dwarfin/SMAD family. SMAD7 Interaction with NEDD4L or RNF111 induces translocation from the nucleus to the cytoplasm (PubMed:16601693). TGF-beta stimulates its translocation from the nucleus to the cytoplasm. PDPK1 inhibits its translocation from the nucleus to the cytoplasm in response to TGF-beta (PubMed:17327236). SMAD7 as antagonist of signaling by TGF-beta type 1 receptor superfamily members has been shown to inhibit TGF-beta and activin signaling by associating with their receptors thus preventing SMAD2 access. SMAD7 functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex and also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. SMAD7 positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

Publications

Species	Application	Title
mouse	WB	Brain Behav Immun; MicroRNA-181c promotes Th17 cell differentiation and mediates experimental autoimmune encephalomyelitis.; Authors - Zimu Zhang
human	WB	Nat Cell Biol; An ARF6-Exportin-5 axis delivers pre-miRNA cargo to tumour microvesicles.; Authors - James W Clancy
human	WB	Nat Commun; K235 acetylation couples with PSPC1 to regulate the m6A demethylation activity of ALKBH5 and tumorigenesis; Authors - Xiao-Lan Zhang
human	WB	Cell Death Differ; REGγ ablation impedes dedifferentiation of anaplastic thyroid carcinoma and accentuates radio-therapeutic response by regulating the Smad7-TGF-β pathway.; Authors - Chan Jiao
mouse	WB	Hypertension; Novel Role for the Immunoproteasome Subunit PSMB10 in Angiotensin II-Induced Atrial Fibrillation in Mice.; Authors - Jing Li
human	WB	Theranostics; Local administration of liposomal-based Srpx2 gene therapy reverses pulmonary fibrosis by blockading fibroblast-to-myofibroblast transition.; Authors - Qi Wang

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