Anticorps Polyclonal de lapin anti-Sclerostin

• Phare
• Validé par KD/KO

Sclerostin Polyclonal Antibody for IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 21933-1-AP

Synonymes

sclerosteosis, Sclerostin, SOST, VBCH

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Galerie de données de validation

• 

  IHC staining of human kidney using 21933-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21933-1-AP (Sclerostin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 21933-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21933-1-AP (Sclerostin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 21933-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 21933-1-AP (Sclerostin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 21933-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 21933-1-AP (Sclerostin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse embryo using 21933-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 21933-1-AP (Sclerostin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse embryo using 21933-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 21933-1-AP (Sclerostin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 21933-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 21933-1-AP (Sclerostin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 21933-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 21933-1-AP (Sclerostin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse embryo using 21933-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 21933-1-AP (Sclerostin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse embryo using 21933-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 21933-1-AP (Sclerostin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 21933-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21933-1-AP (Sclerostin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 21933-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21933-1-AP (Sclerostin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 21933-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Sclerostin antibody (21933-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en IHC: tissu rénal humain, tissu de cancer du sein humain, tissu embryonnaire de souris, tissu pulmonaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 8 publications below
• IF: See 2 publications below

Informations sur le produit

21933-1-AP cible Sclerostin dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Sclerostin Protéine recombinante Ag16567
• Nom complet: sclerosteosis
• Masse moléculaire calculée: 213 aa, 24 kDa
• Numéro d’acquisition GenBank: BC101086
• Symbole du gène: Sclerostin
• Identification du gène (NCBI): 50964
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Sclerostin is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Loss-of-function mutations in this gene are associated with an autosomal-recessive disorder, sclerosteosis, which causes progressive bone overgrowth. A deletion downstream of this gene, which causes reduced sclerostin expression, is associated with a milder form of the disorder called van Buchem disease

Publications

Species	Application	Title
mouse	IF	EMBO Mol Med; Sigma-1 receptor attenuates osteoclastogenesis by promoting ER-associated degradation of SERCA2.; Authors - Xiaoan Wei
rat	WB	Aging (Albany NY); Cyclooxygenase-2/sclerostin mediates TGF-β1-induced calcification in vascular smooth muscle cells and rats undergoing renal failure.; Authors - Fang He
human	WB,IHC	Front Oncol; Sclerostin Suppression Facilitates Uveal Melanoma Progression Through Activating Wnt/β-Catenin Signaling Via Binding to Membrane Receptors LRP5/LRP6.; Authors - Hanqing Wang ; KD Validated
mouse	IHC	Ann Hematol; Higher Sclerostin/SOST expression is associated with lower percentage of circulatory blasts and better prognosis in patients with myelofibrosis.; Authors - Marko Lucijanic
rat	IHC	Clin Oral Investig; Mechanisms of sphingosine-1-phosphate (S1P) signaling on excessive stress-induced root resorption during orthodontic molar intrusion.; Authors - Han Wang
mouse	WB	J Orthop Surg Res; Lysyl oxidase inhibits BMP9-induced osteoblastic differentiation through reducing Wnt/β-catenin via HIF-1a repression in 3T3-L1 cells; Authors - Jie Zhang

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