- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-SNX9
SNX9 Polyclonal Antibody for WB, IP, IF, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, rat, souris et plus (1)
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugaison
Non conjugué
N° de cat : 15721-1-AP
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | cellules HeLa, tissu cardiaque de rat, tissu cardiaque de souris, tissu cardiaque humain, tissu de muscle squelettique de souris |
Résultats positifs en IP | tissu cardiaque de souris |
Résultats positifs en IF/ICC | cellules HeLa, |
Dilution recommandée
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunoprécipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
KD/KO | See 5 publications below |
WB | See 11 publications below |
IHC | See 1 publications below |
IF | See 2 publications below |
Informations sur le produit
15721-1-AP cible SNX9 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris
Réactivité | Humain, rat, souris |
Réactivité citée | Humain, singe, souris |
Hôte / Isotype | Lapin / IgG |
Clonalité | Polyclonal |
Type | Anticorps |
Immunogène | SNX9 Protéine recombinante Ag8440 |
Nom complet | sorting nexin 9 |
Masse moléculaire calculée | 595 aa, 67 kDa |
Poids moléculaire observé | 78 kDa |
Numéro d’acquisition GenBank | BC005022 |
Symbole du gène | SNX9 |
Identification du gène (NCBI) | 51429 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par affinité contre l'antigène |
Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
Sorting nexins are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). They are involved in endocytosis and protein trafficking. SNX9 (Sorting nexin-9, also known as SH3PX1) was originally identified as a protein that interacted with the metalloproteinases ADAM9 and ADAM15. It contains a PX and an Sec homology 3 (SH3) domain. SNX9 functions in clathrin-mediated endocytosis and clathrin-independent, actin-dependent fluid-phase endocytosis (PMID: 17609109). On SDS-PAGE, SNX9 migrates at a molecular weight (70-78 kDa) higher than its calculated molecular mass of 67 kDa (PMID: 15299020; 12952949; 18411244; 15703209).
Protocole
Product Specific Protocols | |
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WB protocol for SNX9 antibody 15721-1-AP | Download protocol |
IF protocol for SNX9 antibody 15721-1-AP | Download protocol |
IP protocol for SNX9 antibody 15721-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Immunity cis-B7:CD28 interactions at invaginated synaptic membranes provide CD28 co-stimulation and promote CD8+ T cell function and anti-tumor immunity | ||
Nat Commun Selective packaging of mitochondrial proteins into extracellular vesicles prevents the release of mitochondrial DAMPs.
| ||
Cancer Discov In vivo role of INPP4B in tumor and metastasis suppression through regulation of PI3K/AKT signaling at endosomes. | ||
Dev Cell Mitochondrial-derived vesicles compensate for loss of LC3-mediated mitophagy.
| ||
Proc Natl Acad Sci U S A Loss of FCHSD1 leads to amelioration of chronic obstructive pulmonary disease. | ||
Oncotarget Sorting nexin 9 (SNX9) is not essential for development and auditory function in mice.
|