Anticorps Polyclonal de lapin anti-SH3PXD2A

• Phare
• Validé par KD/KO

SH3PXD2A Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

14

N° de cat : 18976-1-AP

Synonymes

Adapter protein TKS5, FISH, KIAA0418, SH3 and PX domains 2A, SH3 multiple domains protein 1, SH3MD1, SH3PXD2A, TKS5

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Galerie de données de validation

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  WB analysis of HeLa using 18976-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18976-1-AP (SH3PXD2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 18976-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18976-1-AP (SH3PXD2A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 18976-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18976-1-AP (SH3PXD2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 18976-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 18976-1-AP (SH3PXD2A antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 18976-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 18976-1-AP (SH3PXD2A antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 18976-1-AP

  Immunofluorescent analysis of HepG2 cells using 18976-1-AP (SH3PXD2A antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2
• Résultats positifs en IHC: tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 6 publications below
• IF: See 5 publications below
• CoIP: See 1 publications below

Informations sur le produit

18976-1-AP cible SH3PXD2A dans les applications de WB, IHC, IF/ICC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: SH3 and PX domains 2A
• Masse moléculaire calculée: 125 kDa
• Poids moléculaire observé: 140-150 kDa
• Numéro d’acquisition GenBank: NM_014631
• Symbole du gène: SH3PXD2A
• Identification du gène (NCBI): 9644
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SH3PXD2A is also named as FISH, KIAA0418, SH3MD1, TKS5. SH3PXD2A contains an amino-terminal PX domain followed by five SH3 domains. It is a cytoplasmic protein in normal fibroblasts (PMID: 19464300). The p140 and p130 forms of SH3PXD2A may be generated by other splice variations. The complexity of SH3PXD2A isoforms is also evident in different cell types. For example in human platelets a single band of 150 kDa was detected, whereas all three forms were found in human fibroblasts and vascular smooth muscle cells (PMID: 9687503). This antibody is specific to SH3PXD2A.

Publications

Species	Application	Title
human	WB	Nat Commun; Cytoplasmic LIF reprograms invasive mode to enhance NPC dissemination through modulating YAP1-FAK/PXN signaling.; Authors - Shu-Chen Liu
mouse	IF	Circ. Res.; Inhibition of KLF5-Myo9b-RhoA Pathway-Mediated Podosome Formation in Macrophages Ameliorates Abdominal Aortic Aneurysm.; Authors - Dong Ma
human	IF	Adv Healthc Mater; Fiber Microarchitecture in Interpenetrating Collagen-Alginate Hydrogel with Tunable Mechanical Plasticity Regulates Tumor Cell Migration; Authors - Zhao Wei
		Theranostics; IKKε phosphorylates kindlin-2 to induce invadopodia formation and promote colorectal cancer metastasis.; Authors - Ge Liu
human	WB	Oncotarget; The role of Tks adaptor proteins in invadopodia formation, growth and metastasis of melanoma.; Authors - Shinji Iizuka ; KD Validated
human	WB,IHC,IF	J Oncol; Tumour: Fibroblast Interactions Promote Invadopodia-Mediated Migration and Invasion in Oral Squamous Cell Carcinoma; Authors - Koroku Kato ; KD Validated

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