Anticorps Polyclonal de lapin anti-Septin 9

• Phare
• Validé par KD/KO

Septin 9 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

28

N° de cat : 10769-1-AP

Synonymes

SEPT9, Septin-9, MSF, MLL septin-like fusion protein MSF-A, MLL septin-like fusion protein

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Galerie de données de validation

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  WB analysis using 10769-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10769-1-AP (Septin 9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10769-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10769-1-AP (Septin 9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10769-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10769-1-AP (Septin 9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 10769-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 10769-1-AP (Septin 9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 10769-1-AP

  IP result of anti-Septin 9 (IP:10769-1-AP, 4ug; Detection:10769-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human pancreas cancer using 10769-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10769-1-AP (Septin 9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 10769-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10769-1-AP (Septin 9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 10769-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10769-1-AP (Septin 9 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 10769-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10769-1-AP (Septin 9 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of hTERT-RPE cells using 10769-1-AP

  IF result of anti-Septin 9 (10769-1-AP, 1:200) with hTERT-RPE cell by Moshe Kim (laboratory of Dr. William S Trimble, University of Toronto). Green: SEPT9. Blue: DNA. Fixation in methanol.

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  IF Staining of HepG2 using 10769-1-AP

  Immunofluorescent analysis of HepG2 cells, using Septin 9 antibody 10769-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of A431 using 10769-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Septin 9 antibody (10769-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 10769-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Septin 9 antibody (10769-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 10769-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Septin 9 (10769-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules C6, cellules HeLa, tissu cérébral de rat, tissu cérébral de souris, tissu de thymus de souris, tissu splénique de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du pancréas humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules A431, cellules HepG2, cellules hTERT-RPE
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 20 publications below
• IHC: See 1 publications below
• IF: See 15 publications below

Informations sur le produit

10769-1-AP cible Septin 9 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, canin, Drosophile, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Septin 9 Protéine recombinante Ag1218
• Nom complet: septin 9
• Masse moléculaire calculée: 64 kDa
• Poids moléculaire observé: 65 kDa, 38-40 kDa
• Numéro d’acquisition GenBank: BC021192
• Symbole du gène: SEPT9
• Identification du gène (NCBI): 10801
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SEPT9 belongs to the septin family which is a group of highly conserved GTP-binding proteins in eukaryotes. SEPT9 plays a role in cytokinesis, the final stage of cell division. SEPT9 gene methylation has been implicated as a biomarker for colorectal cancer(CRC).

Publications

Species	Application	Title
rat	IF	Dev Cell; Polarity of Neuronal Membrane Traffic Requires Sorting of Kinesin Motor Cargo during Entry into Dendrites by a Microtubule-Associated Septin.; Authors - Eva P Karasmanis ; KD Validated
canine	IF	Curr Biol; A Septin Double Ring Controls the Spatiotemporal Organization of the ESCRT Machinery in Cytokinetic Abscission.; Authors - Eva P Karasmanis ; KD Validated
human	WB,IF	Curr Biol; Cdc42 and its BORG2 and BORG3 effectors control the subcellular localization of septins between actin stress fibers and microtubules.; Authors - Joëlle Salameh
human	WB, IF	J Cell Biol; A septin GTPase scaffold of dynein-dynactin motors triggers retrograde lysosome transport.; Authors - Ilona A Kesisova
human	IF	J Cell Biol; Septins suppress the release of vaccinia virus from infected cells.; Authors - Julia Pfanzelter
mouse	WB	PLoS Genet; Genetic deletion of SEPT7 reveals a cell type-specific role of septins in microtubule destabilization for the completion of cytokinesis.; Authors - Manoj B Menon

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