Anticorps Polyclonal de lapin anti-SCO2

SCO2 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 21223-1-AP

Synonymes

SCO1L, SCO2

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Galerie de données de validation

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  WB analysis using 21223-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21223-1-AP (SCO2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 21223-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21223-1-AP (SCO2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 21223-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21223-1-AP (SCO2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse skeletal muscle using 21223-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 21223-1-AP (SCO2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 21223-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 21223-1-AP (SCO2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocirrhosis using 21223-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 21223-1-AP (SCO2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 21223-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 21223-1-AP (SCO2 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of mouse skeletal muscle using 21223-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using SCO2 antibody (21223-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HepG2, cellules HUVEC
• Résultats positifs en IHC: tissu de muscle squelettique de souris, tissu cardiaque humain, tissu de cirrhose hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de muscle squelettique de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 10 publications below
• IHC: See 2 publications below

Informations sur le produit

21223-1-AP cible SCO2 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SCO2 Protéine recombinante Ag15663
• Nom complet: SCO cytochrome oxidase deficient homolog 2 (yeast)
• Masse moléculaire calculée: 266 aa, 30 kDa
• Poids moléculaire observé: 27-30 kDa
• Numéro d’acquisition GenBank: BC102025
• Symbole du gène: SCO2
• Identification du gène (NCBI): 9997
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Human SCO2 is a mitochondrial membrane-bound protein involved in copper supply for the assembly of cytochrome c oxidase in eukaryotes.It is involved in the maintenance of cell copper homeostasis or, limited to HSco2, as a downstream mediator of the Warburg effect, as its expression is regulated by p53(PMID:17850752).This protein belongs to the SCO1/2 family and defects in SCO2 are the cause of fatal infantile cardioencephalomyopathy with cytochrome c oxidase deficiency (FIC).

Publications

Species	Application	Title
human	WB	Cell Metab; Cooperation between COA6 and SCO2 in COX2 maturation during cytochrome c oxidase assembly links two mitochondrial cardiomyopathies.; Authors - David Pacheu-Grau
mouse	WB	Cell Death Differ; Mitophagy protein PINK1 suppresses colon tumor growth by metabolic reprogramming via p53 activation and reducing acetyl-CoA production.; Authors - Kunlun Yin
human	WB	EMBO Mol Med; APOPT1/COA8 assists COX assembly and is oppositely regulated by UPS and ROS.; Authors - Alba Signes
human	WB,IHC	Oncotarget; The oncoprotein HBXIP promotes glucose metabolism reprogramming via downregulating SCO2 and PDHA1 in breast cancer.; Authors - Fabao Liu
human	WB,IHC	Int J Mol Sci; SLMP53-1 Inhibits Tumor Cell Growth through Regulation of Glucose Metabolism and Angiogenesis in a P53-Dependent Manner.; Authors - Helena Ramos
mouse	WB	PLoS One; Mitochondrial dysfunction in Pten haplo-insufficient mice with social deficits and repetitive behavior: interplay between Pten and p53.; Authors - Napoli Eleonora E