Anticorps Polyclonal de lapin anti-SCNN1A

• Phare
• Validé par KD/KO

SCNN1A Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

15

N° de cat : 10924-2-AP

Synonymes

Alpha ENaC, Alpha NaCH, ENaCa, ENaCalpha, SCNEA, SCNN1, SCNN1A

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Galerie de données de validation

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  WB analysis of HEK-293 using 10924-2-AP

  WB result of SCNN1A (10924-2-AP, 1:1000) with si-control and si-SCNN1A transfected HEK293 cells.

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  WB analysis of PC-13 using 10924-2-AP

  PC-13 cells were subjected to SDS PAGE followed by western blot with 10924-2-AP (SCNN1A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10924-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10924-2-AP (SCNN1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 10924-2-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10924-2-AP (SCNN1A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 10924-2-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10924-2-AP (SCNN1A Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 10924-2-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10924-2-AP (SCNN1A Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A549 using 10924-2-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 10924-2-AP (SCNN1A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules PC-13, HEK-293
• Résultats positifs en IHC: tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 7 publications below

Informations sur le produit

10924-2-AP cible SCNN1A dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SCNN1A Protéine recombinante Ag1325
• Nom complet: sodium channel, nonvoltage-gated 1 alpha
• Masse moléculaire calculée: 76 kDa
• Poids moléculaire observé: 60-70 kDa
• Numéro d’acquisition GenBank: BC006526
• Symbole du gène: SCNN1A
• Identification du gène (NCBI): 6337
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SCNN1A (sodium channel, non-voltage-gated 1 alpha), also known as ENaCA (epithelial Na(+) channel subunit alpha) or amiloride-sensitive sodium channel subunit alpha, is the alpha subunit of the epithelial Na(+) channel (ENaC). ENaC is expressed in the apical membrane of salt-absorbing epithelia of kidney, distal colon, and lung. ENaC is a non-voltage gated, constitutively active channel highly selective for sodium. It has an essential role in salt and fluid homeostasis across epithelial tissues. ENaC consists of three different subunits: alpha, beta, gamma. Mutations in the gene of SCNN1A have been associated with pseudohypoaldosteronism type 1 (PHA1), a rare salt wasting disease resulting from target organ unresponsiveness to mineralocorticoids. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. It has been reported that full-length SCNN1A protein can be cleaved into 65- and 30-kDa fragments (PMID: 16477034; 18701608).

Publications

Species	Application	Title
human	WB	Am J Respir Crit Care Med; SPX-101 is a Novel ENaC-targeted Therapeutic for Cystic Fibrosis that Restores Mucus Transport.; Authors - David W Scott
mouse	IHC	Sci Adv; MLL4 mediates differentiation and tumor suppression through ferroptosis.; Authors - Shaun Egolf
mouse	WB	Hypertension; Renal Natriuretic Peptide Receptor-C Deficiency Attenuates NaCl Cotransporter Activity in Angiotensin II-Induced Hypertension.; Authors - Shuai Shao
	IHC	Sci Rep; Distribution of aquaporins and sodium transporters in the gastrointestinal tract of a desert hare, Lepus yarkandensis.; Authors - Jianping Zhang
human	WB	Biomed Pharmacother; PMID: 31203134; Authors - Wei-Hua He
human	WB,IHC	PLoS One; Reduced Expression of Enac in Placenta Tissues of Patients with Severe Preeclampsia Is Related to Compromised Trophoblastic Cell Migration and Invasion during Pregnancy.; Authors - Wang Shan S

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