Anticorps Polyclonal de lapin anti-RUNX2

• Phare
• Validé par KD/KO

RUNX2 Polyclonal Antibody for WB, IF/ICC, IF-P, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

133

N° de cat : 20700-1-AP

Synonymes

OSF2, OSF 2, Core-binding factor subunit alpha-1, CBF-alpha-1, AML-3

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Galerie de données de validation

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  WB analysis of ROS1728 using 20700-1-AP

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 20700-1-AP (RUNX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 20700-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 20700-1-AP (RUNX2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of ROS1728 using 20700-1-AP

  IP result of anti-RUNX2 (IP:20700-1-AP, 4ug; Detection:20700-1-AP 1:1000) with ROS1728 cells lysate 2000 ug.

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  IF Staining of mouse brain using 20700-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 20700-1-AP (RUNX2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of ROS1728 using 20700-1-AP

  Immunofluorescent analysis of (4% PFA) fixed ROS1728 cells using RUNX2 antibody (20700-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 20700-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human RUNX2 (20700-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules ROS1728, cellules MCF-7
• Résultats positifs en IP: cellules ROS1728,
• Résultats positifs en IF-P: tissu cérébral de souris,
• Résultats positifs en IF/ICC: cellules ROS1728,
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 118 publications below
• IHC: See 15 publications below
• IF: See 22 publications below
• IP: See 1 publications below
• ChIP: See 3 publications below

Informations sur le produit

20700-1-AP cible RUNX2 dans les applications de WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: runt-related transcription factor 2
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 57-60 kDa
• Numéro d’acquisition GenBank: NM_004348
• Symbole du gène: RUNX2
• Identification du gène (NCBI): 860
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RUNX2, also named as AML3, CBFA1, OSF2 and PEBP2A, is a Transcription factor which involved in osteoblastic differentiation and skeletal morphogenesis. It is essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters By similarity. RUNX2 inhibits MYST4-dependent transcriptional activation. The antibody is specific to RUNX2.

Publications

Species	Application	Title
human	WB	Bioact Mater; 3D Printed Biomimetic Metamaterials with Graded Porosity and Tapering Topology for Improved Cell Seeding and Bone Regeneration; Authors - Lei Zhang
human	WB	Mater Today Bio; Sustained release of naringin from silk-fibroin-nanohydroxyapatite scaffold for the enhancement of bone regeneration.; Authors - Zhi-Hu Zhao
	WB	ACS Appl Mater Interfaces; Synergistic Effect of Magneto-Mechanical Bioengineered Stem Cells and Magnetic Field to Alleviate Osteoporosis; Authors - Congcong Yu
mouse	IHC	Clin Immunol; STING contributes to trauma-induced heterotopic ossification through NLRP3-dependent macrophage pyroptosis; Authors - Ziyang Sun
mouse	WB	Cell Prolif; Effects of tetrahedral DNA nanostructures on the treatment of osteoporosis; Authors - Weitong Cui
mouse	WB,IF	Br J Pharmacol; Hydrogen sulfide inhibits calcification of heart valves; implications for calcific aortic valve disease.; Authors - Katalin Éva Sikura

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