Anticorps Polyclonal de lapin anti-RUFY1

RUFY1 Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 13498-1-AP

Synonymes

FYVE finger protein EIP1, La binding protein 1, Rab4 interacting protein, RABIP4, RUFY1, ZFYVE12

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Galerie de données de validation

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  WB analysis of HeLa using 13498-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13498-1-AP (RUFY1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa cells using 13498-1-AP

  IP result of anti-RUFY1 (13498-1-AP for IP and Detection).

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  IF Staining of HeLa using 13498-1-AP

  Immunofluorescent analysis of Hela cells, using RUFY1 antibody 13498-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: cellules HeLa
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 4 publications below
• CoIP: See 1 publications below

Informations sur le produit

13498-1-AP cible RUFY1 dans les applications de WB, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RUFY1 Protéine recombinante Ag4324
• Nom complet: RUN and FYVE domain containing 1
• Masse moléculaire calculée: 708 aa, 80 kDa
• Poids moléculaire observé: 69 kDa, 80 kDa
• Numéro d’acquisition GenBank: BC032571
• Symbole du gène: RUFY1
• Identification du gène (NCBI): 80230
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Rab proteins form the largest group within the Ras superfamily of small GTPases, which acts as molecular switches by cycling between their inactive active GTP-bound and GDP-bound forms [PMID:15520808]. The small GTP-binding protein Rab4 has been involved in the recycling of αvβ3 integrins in response to platelet-derived growth factor (PDGF) stimulation suggesting a role for Rab4 in cell adhesion and migration [PMID:17001082]. Rabip4 (Rab4 interacting protein), which possesses a RUN domain, two coiled-coil domains and a FYVE finger, was identified as an effector of Rab4, a small GTPase that plays a role in the endocytotic pathway in all cell types [PMID:11172003]. RUFY1 exsits three isoforms and molecular weight of isoforms are 80 kDa,69 kDa and 45 kDa.

Publications

Species	Application	Title
mouse	WB,IF	Cell; Mouse oocytes sequester aggregated proteins in degradative super-organelles; Authors - Gabriele Zaffagnini
human	IF	Nat Commun; Amino acids stimulate the endosome-to-Golgi trafficking through Ragulator and small GTPase Arl5.; Authors - Meng Shi
mouse	IF	Mol Biol Cell; Trafficking defects in WASH-knockout fibroblasts originate from collapsed endosomal and lysosomal networks.; Authors - Gomez Timothy S TS
human	WB, IF	Mol Biol Cell; COMMD1 is linked to the WASH complex and regulates endosomal trafficking of the copper transporter ATP7A.; Authors - Christine A Phillips-Krawczak
human	CoIP	Cancer Sci; Podocalyxin-like protein promotes gastric cancer progression through interacting with RUN and FYVE domain containing 1 protein.; Authors - Qiaoming Zhi