Anticorps Polyclonal de lapin anti-RPS8

RPS8 Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

6

Avis (1)

N° de cat : 18228-1-AP

Synonymes

40S ribosomal protein S8, ribosomal protein S8, RPS8

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Galerie de données de validation

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  WB analysis using 18228-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18228-1-AP (RPS8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SGC-7901 using 18228-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 18228-1-AP (RPS8 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18228-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18228-1-AP (RPS8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 18228-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 18228-1-AP (RPS8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18228-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18228-1-AP (RPS8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 18228-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18228-1-AP (RPS8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 18228-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18228-1-AP (RPS8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 18228-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 18228-1-AP (RPS8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of A375 using 18228-1-AP

  Immunofluorescent analysis of A375 cells using 18228-1-AP (RPS8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A375, cellules PC-3, cellules SGC-7901, tissu hépatique de souris, tissu pancréatique de souris
• Résultats positifs en IF/ICC: cellules A375

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 3 publications below

Informations sur le produit

18228-1-AP cible RPS8 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RPS8 Protéine recombinante Ag12313
• Nom complet: ribosomal protein S8
• Masse moléculaire calculée: 208 aa, 24 kDa
• Poids moléculaire observé: 25-28 kDa
• Numéro d’acquisition GenBank: BC070875
• Symbole du gène: RPS8
• Identification du gène (NCBI): 6202
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Dev Cell; Autophagic elimination of ribosomes during spermiogenesis provides energy for flagellar motility.; Authors - Yuqing Lei
human	IHC	Cancer Biol Med; A novel recurrence-associated metabolic prognostic model for risk stratification and therapeutic response prediction in patients with stage I lung adenocarcinoma.; Authors - Chengming Liu
human	WB	Mol Cell Proteomics; Proteomic landscape of exosomes reveals the functional contributions of CD151 in triple-negative breast cancer.; Authors - Sipeng Li
human	IHC	Lab Invest; Proteins associated with pancreatic cancer survival in patients with resectable pancreatic ductal adenocarcinoma.; Authors - Ru Chen
human	IHC	Oncol Rep; Identification of 40S ribosomal protein S8 as a novel biomarker for alcohol‑associated hepatocellular carcinoma using weighted gene co‑expression network analysis.; Authors - Ningrui Bi
human	WB	Int J Mol Sci; Isolation of Endogenously Assembled RNA-Protein Complexes Using Affinity Purification Based on Streptavidin Aptamer S1.; Authors - Yangchao Dong

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Anita (Verified Customer) (10-30-2020)

Works at 1:1000 dilution for HeLa cells and C. elegans nematodes

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: C. elegans and HeLa cells

RPS8 Antibody Western Blot validation (1:1000 dilution) in C. elegans and HeLa cells (Cat no:18228-1-AP)