Anticorps Polyclonal de lapin anti-RPL13A

RPL13A Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 14633-1-AP

Synonymes

23 kDa highly basic protein, 60S ribosomal protein L13a, ribosomal protein L13a, RPL13A

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Galerie de données de validation

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  WB analysis using 14633-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14633-1-AP (RPL13A antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 14633-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14633-1-AP (RPL13A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14633-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14633-1-AP (RPL13A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293, cellules HeLa, cellules Jurkat, cellules RAW264.7

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

14633-1-AP cible RPL13A dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RPL13A Protéine recombinante Ag6212
• Nom complet: ribosomal protein L13a
• Masse moléculaire calculée: 23 kDa
• Poids moléculaire observé: 25-28 kDa
• Numéro d’acquisition GenBank: BC065236
• Symbole du gène: RPL13A
• Identification du gène (NCBI): 23521
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ribosomal protein L13a(RPL13a) is a member of the L13 ribosomal protein family and a structural component of the large 60S ribosomal subunit. It's a reference gene(RG) that stably expressed not only during differentiation, but also during expansion to higher passages. And it's suggested as a RG for a variety research field. RPL13a is a key component of the IFN-gamma-activated inhibitor of translation(GAIT) complex, which inhibits translation of a select group of inflammation-related transcripts via binding to rhe 3' UTR of target mRNA.once phosphorylated of Ser77 by ZIPK, RPL13a released from the complex.