Anticorps Polyclonal de lapin anti-RGS4

• Phare
• Validé par KD/KO

RGS4 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 14530-1-AP

Synonymes

RGP4, RGS4, SCZD9

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Galerie de données de validation

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  WB analysis of mouse cerebellum using 14530-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 14530-1-AP (RGS4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14530-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14530-1-AP (RGS4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 14530-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 14530-1-AP (RGS4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 14530-1-AP

  IP result of anti-RGS4 (IP:14530-1-AP, 4ug; Detection:14530-1-AP 1:300) with mouse brain tissue lysate 4000ug.

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  IHC staining of human lung using 14530-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 14530-1-AP (RGS4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 14530-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 14530-1-AP (RGS4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 14530-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14530-1-AP (RGS4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 14530-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14530-1-AP (RGS4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 14530-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RGS4 antibody (14530-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu de cervelet de souris, cellules PC-12, tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu pulmonaire humain, tissu cérébral de souris, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

14530-1-AP cible RGS4 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RGS4 Protéine recombinante Ag6029
• Nom complet: regulator of G-protein signaling 4
• Masse moléculaire calculée: 23 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC051869
• Symbole du gène: RGS4
• Identification du gène (NCBI): 5999
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Regulator of G protein signaling (RGS) proteins are a large family of highly diverse, multifunctional signaling proteins. RGS4 is one of seven members of a classic R4 RGS protein that accelerates the GTPase activity of the Gai/o and Gaq/11 family members. RGS4 is highly expressed in multiple brain regions including the cerebral cortex, amygdala, hippocampus, and striatum. Extensive studies have demonstrated that RGS4 plays an important role in regulating smooth muscle contraction, cardiomyocyte development, neural plasticity and psychiatric disorders.

Publications

Species	Application	Title
human,mouse	IHC	J Allergy Clin Immunol; RGS4 promotes allergen- and aspirin-associated airway hyperresponsiveness by inhibiting PGE2 biosynthesis.; Authors - Gordon S Wong ; KO Validated
mouse	WB	Arterioscler Thromb Vasc Biol; HuR (Human Antigen R) Regulates the Contraction of Vascular Smooth Muscle and Maintains Blood Pressure.; Authors - Shanshan Liu
human	WB,IHC	Oncotarget; Regulator of G protein signaling 4 inhibits human melanoma cells proliferation and invasion through the PI3K/AKT signaling pathway.; Authors - Xiaotong Xue
human	WB,IHC,IF	Onco Targets Ther; RGS4 Regulates Proliferation And Apoptosis Of NSCLC Cells Via microRNA-16 And Brain-Derived Neurotrophic Factor.; Authors - Zheng He
human	WB	Arch Gerontol Geriatr; LINC00370 modulates miR-222-3p-RGS4 axis to protect against osteoporosis progression.; Authors - Lintao Li
human	WB	Redox Biol; Redox-modulated SNX25 as a novel regulator of GPCR-G protein signaling from endosomes; Authors - Yulong Zhang