Anticorps Polyclonal de lapin anti-RAP1B

RAP1B Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 10840-1-AP

Synonymes

GTP binding protein smg p21B, K REV, RAL1B, RAP1B, Ras related protein Rap 1b

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Galerie de données de validation

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  WB analysis of A549 using 10840-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10840-1-AP (RAP1B antibody) at dilution of 1:300. .

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  WB analysis using 10840-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10840-1-AP (RAP1B antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

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  WB analysis using 10840-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10840-1-AP (RAP1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10840-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10840-1-AP (RAP1B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10840-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10840-1-AP (RAP1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 10840-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 10840-1-AP (RAP1B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10840-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10840-1-AP (RAP1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human oesophagus cancer using 10840-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10840-1-AP (RAP1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 10840-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 10840-1-AP (RAP1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A431, cellules A549, cellules C6, cellules HepG2, cellules NIH/3T3, tissu cérébral de souris, tissu cérébral humain, tissu rénal de souris
• Résultats positifs en IHC: tissu de cancer de l'œsophage humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

10840-1-AP cible RAP1B dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, poulet
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAP1B Protéine recombinante Ag1282
• Nom complet: RAP1B, member of RAS oncogene family
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 21 kDa
• Numéro d’acquisition GenBank: BC000176
• Symbole du gène: RAP1B
• Identification du gène (NCBI): 5908
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RAP1B is a member of the RAS-like small GTP-binding protein superfamily. Members of this family regulate multiple cellular processes including cell adhesion and growth and differentiation. RAP1B localizes to cellular membranes and has been shown to regulate integrin-mediated cell signaling. It also plays a role in regulating outside-in signaling in platelets.

Publications

Species	Application	Title
human	WB	Mol Cell; Macrophage-Associated PGK1 Phosphorylation Promotes Aerobic Glycolysis and Tumorigenesis.; Authors - Yajuan Zhang
human	WB	Oncotarget; LncRNA MT1JP functions as a tumor suppressor by interacting with TIAR to modulate the p53 pathway.; Authors - Lihui Liu
human	WB	Int J Mol Sci; Molecular and Pharmacological Characterization of the Interaction between Human Geranylgeranyltransferase Type I and Ras-Related Protein Rap1B.; Authors - Sonja Hinz
chicken	WB,IP	Microbiol Spectr; Avian Hepatitis E Virus ORF2 Protein Interacts with Rap1b to Induce Cytoskeleton Rearrangement That Facilitates Virus Internalization.; Authors - Beibei Zhang
human	WB,IF	Viruses; ICP4-Associated Activation of Rap1b Facilitates Herpes Simplex Virus Type I (HSV-1) Infection in Human Corneal Epithelial Cells; Authors - Beibei Zhang
human	WB	Int J Biochem Cell Biol; Regulation of RAP1B by miR-139 suppresses human colorectal carcinoma cell proliferation.; Authors - Guo Haiyan H