Anticorps Polyclonal de lapin anti-PICK1

• Phare
• Validé par KD/KO

PICK1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

9

Avis (1)

N° de cat : 10983-2-AP

Synonymes

PICK, PICK1, PRKCA binding protein, PRKCABP

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Galerie de données de validation

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  WB analysis using 10983-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10983-2-AP (PICK1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 10983-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10983-2-AP (PICK1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10983-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10983-2-AP (PICK1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 10983-2-AP

  IP result of anti-PICK1 (IP:10983-2-AP, 4ug; Detection:10983-2-AP 1:2500) with mouse brain tissue lysate 1280 ug.

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  IHC staining of human stomach cancer using 10983-2-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10983-2-AP (PICK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 10983-2-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10983-2-AP (PICK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 10983-2-AP

  Immunofluorescent analysis of HEK-293 cells using 10983-2-AP (PICK1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules SH-SY5Y, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Informations sur le produit

10983-2-AP cible PICK1 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PICK1 Protéine recombinante Ag1443
• Nom complet: protein interacting with PRKCA 1
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC017561
• Symbole du gène: PICK1
• Identification du gène (NCBI): 9463
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Protein interacting with C kinase 1 (PICK1) was first cloned as a PKC-binding partner through yeast two hybrid system. PICK1 acts as a critical regulator of membrane receptors' subcellular trafficking to modulate neural processes such as learning and memory, and is widely expressed in brain, testis, heart, lung, liver, kidney and muscle. It probably binds to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence, for instance, PICK1 is a critical mediator of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) trafficking in neural synapses. PICK1 expression on D-serine release and glutamate transport in astrocytes suggests a potential implication of PICK1 in the progression of amyotrophic lateral sclerosis (ALS). PICK1 may also participate in breast cancer development through inhibition of TGF-β signaling.

Publications

Species	Application	Title
human	WB,IHC	Cell Res; PICK1 promotes caveolin-dependent degradation of TGF-β type I receptor.; Authors - Zhao Bing B ; KO Validated
mouse	WB	Environ Int; Repression of autophagy leads to acrosome biogenesis disruption caused by a sub-chronic oral administration of polystyrene nanoparticles.; Authors - Lixiao Zhou
mouse	IF	Front Neuroanat; Deletion of KIBRA, protein expressed in kidney and brain, increases filopodial-like long dendritic spines in neocortical and hippocampal neurons in vivo and in vitro.; Authors - Anja Blanque
human	WB,IHC	Cancer Sci; Protein interacting with C alpha kinase 1 (PICK1) is involved in promoting tumor growth and correlates with poor prognosis of human breast cancer.; Authors - Zhang Bin B
human		Acta Pharm; Inhibitory effect of taspine derivative TAD1822-7 on tumor cell growth and angiogenesis via suppression of EphrinB2 and related signaling pathways.; Authors - Rui Liu
human	WB	Phytomedicine; Berberine inhibits the proliferation and migration of breast cancer ZR-75-30 cells by targeting Ephrin-B2.; Authors - Weina Ma

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Ambra (Verified Customer) (01-20-2021)

I used this rabbit anti-Pick 1:1000 in 5% milk in TBS-T on lysates from human brain (temporal cortex).NOTE: 1 clear band is visible using a 15% acrylamide gel. Lower gel percentage (10%) give a duplet band.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human brain tissue

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