Anticorps Monoclonal anti-PD-1/CD279

PD-1/CD279 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

30

CloneNo.

4H4D1

Avis (3)

N° de cat : 66220-1-Ig

Synonymes

CD279, hPD 1, hPD l, PD1, PD-1, PD-1/CD279, PDCD1, programmed cell death 1, Protein PD 1, SLEB2

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Galerie de données de validation

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  WB analysis using 66220-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66220-1-Ig (PD-1/CD279 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

• 

  WB analysis using 66220-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66220-1-Ig (PD-1/CD279 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

• 

  WB analysis of mouse thymus using 66220-1-Ig

  mouse thymus tissue was subjected to SDS PAGE followed by western blot with 66220-1-Ig (PD-1/CD279 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat spleen using 66220-1-Ig

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 66220-1-Ig (PD-1/CD279 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lymph node using 66220-1-Ig

  human lymph node tissue were subjected to SDS PAGE followed by western blot with 66220-1-Ig (PD-1/CD279 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 66220-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66220-1-Ig (PD-1/CD279 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66220-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66220-1-Ig (PD-1/CD279 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66220-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66220-1-Ig (PD-1/CD279 antibody) at dilution of 1:4000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 66220-1-Ig

  Immunohistochemical analysis of paraffin-embedded human angioimmunoblastic T-cell lymphoma tissue slide using 66220-1-Ig (PD-1/CD279 Antibody) at dilution of 1:200 (under 10x lens). . Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0.

• 

  IHC staining of human lymphoma using 66220-1-Ig

  Immunohistochemical analysis of paraffin-embedded human angioimmunoblastic T-cell lymphoma tissue slide using 66220-1-Ig (PD-1/CD279 Antibody) at dilution of 1:200 (under 40x lens). . Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0.

• 

  IHC staining of human tonsillitis using 66220-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66220-1-Ig (PD-1/CD279 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0.

• 

  IHC staining of human tonsillitis using 66220-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66220-1-Ig (PD-1/CD279 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0.

• 

  IHC staining of human tonsillitis using 66220-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66220-1-Ig (PD-1/CD279 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66220-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66220-1-Ig (PD-1/CD279 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 66220-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using PD-1/CD279 antibody (66220-1-Ig, Clone: 4H4D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human tonsillitis using 66220-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using PD-1/CD279 mouse mAb (66220-1-Ig) at dilution of 1:50 and CD20 rabbit pAb (24828-1-AP) at dilution of 1:50, further stained with Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) for 66220-1-Ig, and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) for 24828-1-AP.

• 

  IF Staining of human tonsillitis using 66220-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using PD-1/CD279 mouse mAb (66220-1-Ig) at dilution of 1:50 and CD20 rabbit pAb (24828-1-AP) at dilution of 1:50, further stained with Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) for 66220-1-Ig, and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) for 24828-1-AP.

• 

  FC experiment of MOLT-4 using 66220-1-Ig

  1X10^6 unstimulated (dashed line) or PMA and ionomycin treated (red) MOLT-4 cells were surface stained with 0.2 ug Anti-Human PD-1/CD279 (66220-1-Ig, Clone: 4H4D1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000, or 0.2 ug isotype control antibody (blue, solid line). Cells were not fixed.

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Applications testées

• Résultats positifs en WB: cellules RAW 264.7, cellules Jurkat, cellules MOLT-4, cellules THP-1, tissu de ganglion lymphatique humain, tissu de thymus de porc, tissu de thymus de souris, tissu splénique de rat
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu d'amygdalite humain,
• Résultats positifs en cytométrie: PMA and ionomycin treated MOLT-4 cells

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 18 publications below
• IHC: See 10 publications below
• IF: See 10 publications below
• FC: See 1 publications below

Informations sur le produit

66220-1-Ig cible PD-1/CD279 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PD-1/CD279 Protéine recombinante Ag12470
• Nom complet: programmed cell death 1
• Masse moléculaire calculée: 288 aa, 32 kDa
• Poids moléculaire observé: 32 kDa, 47-55 kDa
• Numéro d’acquisition GenBank: BC074740
• Symbole du gène: PDCD1
• Identification du gène (NCBI): 5133
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436). The calculated molecular weight of PD-1 is 32 kDa. It has been reported that PD-1 is heavily glycosylated and migrates with an apparent molecular mass of 47-55 kDa on SDS-PAGE (PMID: 8671665; 17640856; 17003438).

Publications

Species	Application	Title
human,mouse	IHC,IF	Nat Commun; ERK and USP5 govern PD-1 homeostasis via deubiquitination to modulate tumor immunotherapy; Authors - Xiangling Xiao
human	WB	Nat Commun; m6A mRNA demethylase FTO regulates melanoma tumorigenicity and response to anti-PD-1 blockade.; Authors - Seungwon Yang
mouse	WB	Cancers (Basel); Overcoming PD-1 Inhibitor Resistance with a Monoclonal Antibody to Secreted Frizzled-Related Protein 2 in Metastatic Osteosarcoma.; Authors - Patrick Nasarre
human,mouse	WB,IHC	Biochem Pharmacol; Targeting HMGA1 contributes to immunotherapy in aggressive breast cancer while suppressing EMT; Authors - Xing Chang
human	WB	Aging (Albany NY); Elevated lymphocyte specific protein 1 expression is involved in the regulation of leukocyte migration and immunosuppressive microenvironment in glioblastoma.; Authors - Jing-Yuan Cao
mouse	WB, IF	CNS Neurosci Ther; The immunomodulatory effect of microglia on ECM neuroinflammation via the PD-1/PD-L1 pathway.; Authors - Yan Shen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Wiesława (Verified Customer) (12-08-2022)

works very well

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000 overnight
• Cell Tissue Type: human cell lines (25 μg): HL-60, NK-92; mouse thymus (25-45 μg)

PD-1/CD279 Antibody Western Blot validation (1:5000 overnight dilution) in human cell lines (25 μg): HL-60, NK-92; mouse thymus (25-45 μg) (Cat no:66220-1-Ig)

FH

Macarena Lucia (Verified Customer) (10-17-2022)

clear band

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

FH

Mona (Verified Customer) (10-17-2021)

Staining of paraffin-embedded lung tissue from hamster. Antigen retrieval was performed with Tris-EDTA (pH 9)

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: hamster (lung)

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