TIM3 Monoclonal antibody

TIM3 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

20

CloneNo.

4C4G3

Reviews (1)

Cat no : 60355-1-Ig

Synonyms

CD366, HAVcr 2, HAVCR2, KIM 3, T cell membrane protein 3, Tim 3, TIM3, TIM-3, TIMD 3, TIMD3

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Validation Data Gallery

• 

  WB analysis of Daudi using 60355-1-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 60355-1-Ig (TIM3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig thymus using 60355-1-Ig

  pig thymus tissue were subjected to SDS PAGE followed by western blot with 60355-1-Ig (TIM3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of ROS1728 using 60355-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 60355-1-Ig (TIM3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 60355-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 60355-1-Ig (TIM3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 60355-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60355-1-Ig (TIM3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 60355-1-Ig

  rat heart tissue were subjected to SDS PAGE followed by western blot with 60355-1-Ig (TIM3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 60355-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 60355-1-Ig (TIM3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Daudi cells, PC-3 cells, pig thymus tissue, ROS1728 cells, human heart tissue, RAW 264.7 cells, rat heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 9 publications below
• FC: See 1 publications below

Product Information

60355-1-Ig targets TIM3 in WB, ELISA applications and shows reactivity with human, pig, mouse, rat samples.

• Tested Reactivity: human, pig, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TIM3 fusion protein Ag16901
• Full Name: hepatitis A virus cellular receptor 2
• Calculated Molecular Weight: 301 aa, 33 kDa
• Observed Molecular Weight: 33 kDa, 50-70 kDa
• GenBank Accession Number: BC020843
• Gene Symbol: TIM3
• Gene ID (NCBI): 84868
• RRID: AB_2881464
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TIM3, also known as HAVCR2, is a member of the recently discovered T cell Ig and mucin domain-containing molecule superfamily. TIM3 is a negative regulatory molecule that is important for T cell tolerance and has a crucial role in autoimmunity and T cell exhaustion during chronic viral infection (PMID: 23180819). TIM3 is expressed by T-helper type 1 (Th1) cells, macrophage, monocyte, dendritic cells, CD8+ T cell and other lymphocyte subsets. Galectin-9 is a ligand for TIM3. TIM3-galectin-9 pathway negatively regulates T helper type 1 immunity (PMID: 16286920). TIM3 is a 280-aa membrane protein with a calculated molecular weight of 33 kDa, the higher molecular weights between 50 and 70 kDa detected by this monoclonal antibody probably represent glycosylated TIM3 (PMID: 20107545; 17069754; 11725301).

Publications

Species	Application	Title
human		EBioMedicine; Immunological profiles of human oligodendrogliomas define two distinct molecular subtypes; Authors - Fan Wu
human	WB	Oncogene; Tumor cell-intrinsic Tim-3 promotes liver cancer via NF-κB/IL-6/STAT3 axis.; Authors - Hualin Zhang
rat	WB	Ecotoxicol Environ Saf; Expression levels of key immune indicators and immune checkpoints in manganese-exposed rats; Authors - Yuanmeng Qi
human		J Pathol; Molecular subtyping reveals immune alterations in IDH wild-type lower-grade diffuse glioma.; Authors - Fan Wu
human		Front Cell Dev Biol; Aberrantly Expressed Galectin-9 Is Involved in the Immunopathogenesis of Anti-MDA5-Positive Dermatomyositis-Associated Interstitial Lung Disease.; Authors - Lin Liang
pig		Front Immunol; Up-Regulation of Immune Checkpoints in the Thymus of PRRSV-1-Infected Piglets in a Virulence-Dependent Fashion.; Authors - Inés Ruedas-Torres

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Santhosh (Verified Customer) (04-15-2019)

Human Tonsil FFPE tissue. Heat mediated antigen retrieval (pH 6)

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:200

TIM3 Antibody Immunohistochemistry, validation (1:200 dilution) in (Cat no:60355-1-Ig)

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