Anticorps Polyclonal de lapin anti-NSFL1C

• Phare
• Validé par KD/KO

NSFL1C Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

5

Avis (1)

N° de cat : 15620-1-AP

Synonymes

NSFL1 (p97) cofactor (p47), NSFL1 cofactor p47, NSFL1C, p47, p97 cofactor p47, UBX1, UBXD10, UBXN2C

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Galerie de données de validation

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  WB analysis of A431 using 15620-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 15620-1-AP (NSFL1C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15620-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15620-1-AP (NSFL1C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15620-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15620-1-AP (NSFL1C Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15620-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15620-1-AP (NSFL1C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 15620-1-AP

  IP result of anti-NSFL1C (IP:15620-1-AP, 4ug; Detection:15620-1-AP 1:1000) with HEK-293 cells lysate 2640ug.

• 

  IHC staining of human lung cancer using 15620-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15620-1-AP (NSFL1C Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 15620-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15620-1-AP (NSFL1C Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 15620-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15620-1-AP (NSFL1C Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 15620-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15620-1-AP (NSFL1C Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HEK-293, tissu hépatique de souris
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 1 publications below

Informations sur le produit

15620-1-AP cible NSFL1C dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NSFL1C Protéine recombinante Ag8040
• Nom complet: NSFL1 (p97) cofactor (p47)
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 47 kDa, 41 kDa
• Numéro d’acquisition GenBank: BC002801
• Symbole du gène: NSFL1C
• Identification du gène (NCBI): 55968
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Proc Natl Acad Sci U S A; Altered cofactor regulation with disease-associated p97/VCP mutations.; Authors - Xiaoyi Zhang
human	WB	Cell Chem Biol; Site-Specific Photo-Crosslinking Proteomics Reveal Regulation of IFITM3 Trafficking and Turnover by VCP/p97 ATPase.; Authors - Xiaojun Wu
human	WB	J Biol Chem; Pathogenic mutations in the Valosin-containing protein/p97(VCP) N-domain inhibit the SUMOylation of VCP and lead to impaired stress response.; Authors - Tao Wang
human	WB,IF	J Cell Sci; The p97-UBXN1 complex regulates aggresome formation.; Authors - Sirisha Mukkavalli ; KD Validated
mouse	WB	Evid Based Complement Alternat Med; Shenxian-Shengmai Oral Liquid Improves Sinoatrial Node Dysfunction through the PKC/NOX-2 Signaling Pathway.; Authors - Heng Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Julia (Verified Customer) (07-02-2019)

Clean and specific bands on Western blot at correct MW.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human