Anticorps Polyclonal de lapin anti-NLRX1

• Phare
• Validé par KD/KO

NLRX1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

19

N° de cat : 17215-1-AP

Synonymes

Caterpiller protein 11.3, CLR11.3, DLNB26, NLR family member X1, NLRX1, NOD26, NOD5, NOD9

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Galerie de données de validation

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  WB analysis using 17215-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17215-1-AP (NLRX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 17215-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17215-1-AP (NLRX1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 17215-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17215-1-AP (NLRX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17215-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17215-1-AP (NLRX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 17215-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17215-1-AP (NLRX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 17215-1-AP

  IP result of anti-NLRX1 (IP:17215-1-AP, 3ug; Detection:17215-1-AP 1:800) with mouse heart tissue lysate 3520ug.

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  IHC staining of human heart using 17215-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 17215-1-AP (NLRX1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 17215-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 17215-1-AP (NLRX1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse heart using 17215-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 17215-1-AP (NLRX1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 17215-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 17215-1-AP (NLRX1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skeletal muscle using 17215-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17215-1-AP (NLRX1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skeletal muscle using 17215-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17215-1-AP (NLRX1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver using 17215-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 17215-1-AP (NLRX1 Antibody) at dilution of 1:50.

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  IHC staining of human liver using 17215-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 17215-1-AP (NLRX1 Antibody) at dilution of 1:50.

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  IF Staining of HepG2 using 17215-1-AP

  Immunofluorescent analysis of HepG2 cells, using NLRX1 antibody 17215-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HepG2 using 17215-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human NLRX1 (17215-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, cellules MCF-7, cellules THP-1, tissu cardiaque de rat, tissu cardiaque de souris, tissu de côlon de rat, tissu de côlon de souris, tissu de muscle squelettique de souris
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IHC: tissu cardiaque humain, tissu cardiaque de souris, tissu de muscle squelettique humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2
• Résultats positifs en FC (Intra): cellules HepG2
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 7 publications below
• WB: See 18 publications below
• IHC: See 3 publications below
• IF: See 6 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

17215-1-AP cible NLRX1 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NLRX1 Protéine recombinante Ag11000
• Nom complet: NLR family member X1
• Masse moléculaire calculée: 975 aa, 108 kDa
• Poids moléculaire observé: 100-110 kDa
• Numéro d’acquisition GenBank: BC013199
• Symbole du gène: NLRX1
• Identification du gène (NCBI): 79671
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Nature; Mitochondrial DNA stress primes the antiviral innate immune response.; Authors - A Phillip West
human	WB	Mol Cell; Mitochondrial protein import stress regulates the LC3 lipidation step of mitophagy through NLRX1 and RRBP1.; Authors - Samuel A Killackey ; KD Validated
human	WB,IHC	J Exp Med; NLRX1 dampens oxidative stress and apoptosis in tissue injury via control of mitochondrial activity.; Authors - Geurt Stokman ; KO Validated
human,mouse	WB	PLoS Pathog; PMID: 28542569; Authors - Jae-Hoon Kim
human	WB	Front Immunol; PMID: 30344524; Authors - Tünde Fekete
mouse	IF	Front Immunol; NLRX1 Facilitates Histoplasma capsulatum-Induced LC3-Associated Phagocytosis for Cytokine Production in Macrophages.; Authors - Juin-Hua Huang

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