NFKB1,p105,p50-Specific Polyclonal antibody

• Featured Product
• KD/KO Validated

NFKB1,p105,p50-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

21

Cat no : 15506-1-AP

Synonyms

NFKB1, NF kappa B, KBF1, EBP-1, EBP 1

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Validation Data Gallery

• 

  WB analysis of A431 using 15506-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 15506-1-AP (NFKB1,p105,p50-Specific antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 15506-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 15506-1-AP (NFKB1,p105,p50-Specific antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 15506-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 15506-1-AP (NFKB1,p105,p50-Specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A431 cells, Raji cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 20 publications below
• IHC: See 1 publications below

Product Information

15506-1-AP targets NFKB1,p105,p50-Specific in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
• Calculated Molecular Weight: 105 kDa
• Observed Molecular Weight: 50 kDa, 105 kDa
• GenBank Accession Number: NM_003998
• Gene Symbol: NFKB1
• Gene ID (NCBI): 4790
• RRID: AB_2298626
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NFkB is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NFkB is is activated by various intra and extra cellular stimuli such as cytokines, oxidant free radicals, ultraviolet irradiation, and bacterial or viral products. NFkB is a family of transcription factors that consists of homo and heterodimers of NFkB1/p50 and RelA/p65 subunits, and controls a variety of cellular events including development and immune responses. All members share a conserved amino terminus domain that includes dimerization, nuclear localization, and DNA binding regions, and a carboxy terminal transactivation domain. Serines 529 and 536 in the transactivation domain of RelA/p65 are phosphorylated in response to several stimuli including phorbol ester, IL1 alpha and TNF alpha as mediated by IkB kinase and p38 MAPK. Phosphorylation of serines 529 and 536 is critical for RelA/p65 transcriptional activity. Activated NFkB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFkB has been associated with a number of inflammatory diseases while persistent inhibition of NFkB leads to inappropriate immune cell development or delayed cell growth. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. This antibody can bind both p105 and p50 isoforms of NFKB1.

Publications

Species	Application	Title
rat	WB,IHC	Oxid Med Cell Longev; Gastric Electrical Pacing Reduces Apoptosis of Interstitial Cells of Cajal via Antioxidative Stress Effect Attributing to Phenotypic Polarization of M2 Macrophages in Diabetic Rats.; Authors - Hongcai Wang
human	WB	Oncogene; S100A14 suppresses metastasis of nasopharyngeal carcinoma by inhibition of NF-kB signaling through degradation of IRAK1.; Authors - Dong-Fang Meng
human		Pharmacol Res; Chip-DSF: A rapid screening strategy for drug protein targets.; Authors - Zhao Cui
human	WB	ACS Chem Neurosci; Baicalein Delays H2O2-Induced Astrocytic Senescence through Inhibition of Senescence-Associated Secretory Phenotype (SASP), Suppression of JAK2/STAT1/NF-κB Pathway, and Regulation of Leucine Metabolism.; Authors - Li Gao
rat	WB	Int J Mol Sci; Rice Protein Exerts Anti-Inflammatory Effect in Growing and Adult Rats via Suppressing NF-κB Pathway.; Authors - Zhengxuan Wang
mouse	WB	J Ethnopharmacol; Ophiocordyceps lanpingensis polysaccharides alleviate chronic kidney disease through MAPK/NF-κB pathway.; Authors - Shubo Zhou