Anticorps Polyclonal de lapin anti-Nidogen 2

Nidogen 2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 13530-1-AP

Synonymes

NID 2, NID2, Nidogen 2, nidogen 2 (osteonidogen), Osteonidogen

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Galerie de données de validation

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  WB analysis of human placenta using 13530-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13530-1-AP (Nidogen 2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis using 13530-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13530-1-AP (Nidogen 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 13530-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13530-1-AP (Nidogen 2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 13530-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 13530-1-AP (Nidogen 2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 13530-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 13530-1-AP (Nidogen 2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse skeletal muscle using 13530-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 13530-1-AP (Nidogen 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 13530-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 13530-1-AP (Nidogen 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 13530-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13530-1-AP (Nidogen 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 13530-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13530-1-AP (Nidogen 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, tissu cardiaque humain, tissu de muscle squelettique de souris, tissu placentaire humain
• Résultats positifs en IHC: tissu rénal humain, tissu de muscle squelettique de souris, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

13530-1-AP cible Nidogen 2 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Nidogen 2 Protéine recombinante Ag4424
• Nom complet: nidogen 2 (osteonidogen)
• Masse moléculaire calculée: 1375 aa, 151 kDa
• Poids moléculaire observé: 200 kDa
• Numéro d’acquisition GenBank: BC035608
• Symbole du gène: NID2
• Identification du gène (NCBI): 22795
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Nidogen, also referred to as entactin, represents a highly conserved protein in vertebrates and invertebrates and is found in almost all basement membranes (BM). Nidogen-1 (150 kDa) and nidogen-2 (200 kDa) are elongated molecules composed of three globular domains (G1, G2, and G3) connected by a flexible, protease-sensitive link and a rigid rod-like domain. Western blot analysis of protein extracts of heart, lung, and skeletal muscle of mice, major bands of about 200 kDa were detected, accompanied by smaller and variable bands of 160-170 kDa. (PMID: 12215539)

Publications

Species	Application	Title
human	IHC	Front Cell Dev Biol; Time-Resolved Extracellular Matrix Atlas of the Developing Human Skin Dermis.; Authors - Mansheng Li
human	WB,IF	Int Immunopharmacol; Identification of TAP1 as a T-cell related therapeutic target in gastric cancer by mediating oxalipliatin-related synergistic enhancement of immunotherapy; Authors - Yupeng Zhao