Anticorps Polyclonal de lapin anti-NDUFA4L2

• Phare
• Validé par KD/KO

NDUFA4L2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

22

Avis (2)

N° de cat : 16480-1-AP

Synonymes

NDUFA4L2, NUOMS

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Galerie de données de validation

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  WB analysis using 16480-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16480-1-AP (NDUFA4L2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16480-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16480-1-AP (NDUFA4L2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 16480-1-AP

  IP result of anti-NDUFA4L2 (IP:16480-1-AP, 4ug; Detection:16480-1-AP 1:500) with HeLa cells lysate 1200ug.

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  IHC staining of human liver cancer using 16480-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16480-1-AP (NDUFA4L2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 16480-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16480-1-AP (NDUFA4L2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 16480-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16480-1-AP (NDUFA4L2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 16480-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16480-1-AP (NDUFA4L2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 16480-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16480-1-AP (NDUFA4L2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 16480-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16480-1-AP (NDUFA4L2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 16480-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16480-1-AP (NDUFA4L2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 16480-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16480-1-AP (NDUFA4L2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 16480-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16480-1-AP (NDUFA4L2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 16480-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16480-1-AP (NDUFA4L2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 16480-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NDUFA4L2 antibody (16480-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules HeLa, cellules HepG2, cellules HUVEC, cellules PC-3
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu hépatique humain, tissu rénal de souris, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 8 publications below
• IHC: See 9 publications below
• IF: See 12 publications below
• IP: See 1 publications below

Informations sur le produit

16480-1-AP cible NDUFA4L2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NDUFA4L2 Protéine recombinante Ag9600
• Nom complet: NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 4-like 2
• Masse moléculaire calculée: 87 aa, 10 kDa
• Poids moléculaire observé: 10 kDa
• Numéro d’acquisition GenBank: BC011910
• Symbole du gène: NDUFA4L2
• Identification du gène (NCBI): 56901
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NDUFA4L2(NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4-like 2), also named as NUOMS, is a HIF-1α target gene that localizes to the mitochondria and belongs to the complex I NDUFA4 subunit family. It downregulates oxygen consumption and Complex I activity in hypoxia. NDUFA4L2 is involved in hypoxic adaptation by decreasing mitochondrial ROS production.

Publications

Species	Application	Title
human	IF	Adv Sci (Weinh); Single-Cell Transcriptomic Analysis of Primary and Metastatic Tumor Ecosystems in Esophageal Squamous Cell Carcinoma; Authors - Yongxu Jia
human	WB	Cell Metab; Induction of the mitochondrial NDUFA4L2 protein by HIF-1α decreases oxygen consumption by inhibiting Complex I activity.; Authors - Daniel Tello ; KD Validated
human	WB,IHC,IF,IP	Ther Adv Med Oncol; NDUFA4L2 promotes trastuzumab resistance in HER2-positive breast cancer.; Authors - Yuan Yuan
mouse	IF	Sci Signal; Acute O2 sensing through HIF2α-dependent expression of atypical cytochrome oxidase subunits in arterial chemoreceptors.; Authors - Alejandro Moreno-Domínguez ; KO Validated
rat	WB	Basic Res Cardiol; Metabolic adaptation to chronic hypoxia in cardiac mitochondria.; Authors - Heather Lisa C LC
human,rat	WB	Nutrients; Diosgenin Inhibits ROS Generation by Modulating NOX4 and Mitochondrial Respiratory Chain and Suppresses Apoptosis in Diabetic Nephropathy; Authors - Yujie Zhong

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Josh (Verified Customer) (10-28-2021)

Clear, good signal at expected MW (10kDa) by western blot. Non specific bands at 50 and 70 kDa (and to a lesser extent, 15 and 100 kDa).

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: clear cell renal cell carcinoma cell line RCC4

NDUFA4L2 Antibody Western Blot validation (1:1000 dilution) in clear cell renal cell carcinoma cell line RCC4 (Cat no:16480-1-AP)

FH

Daniel (Verified Customer) (09-30-2019)

Kidney cancer mouse model stained for Ndufa4l2 (HRP detection by DAB stain).

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Mouse kidney (TRACK model)

NDUFA4L2 Antibody Immunohistochemistry, validation (1:200 dilution) in Mouse kidney (TRACK model) (Cat no:16480-1-AP)