Anticorps Polyclonal de lapin anti-NAT10

• Phare
• Validé par KD/KO

NAT10 Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA

Conjugaison

Non conjugué

Publications

52

Avis (3)

N° de cat : 13365-1-AP

Synonymes

ALP, KIAA1709, N acetyltransferase 10, NAT10, NET43

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Galerie de données de validation

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  WB analysis of HeLa using 13365-1-AP

  WB result of NAT10 antibody (13365-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NAT10 transfected HeLa cells.

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  WB analysis using 13365-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13365-1-AP (NAT10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 13365-1-AP

  WB result of NAT10 antibody (13365-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NAT10 transfected A549 cells.

• 

  IP experiment of mouse testis using 13365-1-AP

  IP result of anti-NAT10 (IP:13365-1-AP, 4ug; Detection:13365-1-AP 1:8000) with mouse testis tissue lysate 1120 ug.

• 

  IF Staining of HeLa using 13365-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NAT10 antibody (13365-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 13365-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NAT10 antibody (13365-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 13365-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NAT10 antibody (13365-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules NIH/3T3
• Résultats positifs en IP: tissu testiculaire de souris,
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 12 publications below
• WB: See 41 publications below
• IHC: See 8 publications below
• IF: See 14 publications below
• IP: See 2 publications below
• CoIP: See 3 publications below
• ChIP: See 1 publications below
• RIP: See 5 publications below

Informations sur le produit

13365-1-AP cible NAT10 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NAT10 Protéine recombinante Ag4184
• Nom complet: N-acetyltransferase 10 (GCN5-related)
• Masse moléculaire calculée: 1025 aa, 116 kDa
• Poids moléculaire observé: 116 kDa
• Numéro d’acquisition GenBank: BC035558
• Symbole du gène: NAT10
• Identification du gène (NCBI): 55226
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NAT10 (N-acetyltransferase 10) is a nucleolar protein that is involved in regulation of telomerase activity, DNA damage response, and cytokinesis. It also plays a role in maintaining nuclear shape. Inhibition of NAT10 has been reported to rescue the misshapen nuclei in laminopathic cells via microtubule reorganization. The specificity of this antibody has been tested by siRNA. (24786082)

Publications

Species	Application	Title
human	WB,IP	Cell; Acetylation of Cytidine in mRNA Promotes Translation Efficiency.; Authors - Daniel Arango ; KO Validated
human	WB, IF	Science; Chemical inhibition of NAT10 corrects defects of laminopathic cells.; Authors - Delphine Larrieu ; KD Validated
human	WB	Cell Host Microbe; Acetylation of Cytidine Residues Boosts HIV-1 Gene Expression by Increasing Viral RNA Stability.; Authors - Kevin Tsai ; KO Validated
human	WB	Mol Cell; Direct epitranscriptomic regulation of mammalian translation initiation through N4-acetylcytidine.; Authors - Daniel Arango
mouse	WB	Nat Commun; Targeting of NAT10 enhances healthspan in a mouse model of human accelerated aging syndrome.; Authors - Gabriel Balmus ; KO Validated
mouse	WB,IF	Nucleic Acids Res; NAT10-mediated N4-acetylcytidine modification is required for meiosis entry and progression in male germ cells.; Authors - Lu Chen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

SONAM (Verified Customer) (12-18-2023)

Antibody works really well. No Nonspecific bindings.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: cancer cellls

FH

Mattia (Verified Customer) (09-12-2019)

Clear band at the right size, when antibody used at 1:1500 dilution in 5% milk an incubated at 2hrs at 25C. Band is a bit streaky, may require further optimisation.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1500
• Cell Tissue Type: HeLa, HEK293T

FH

Jonathan (Verified Customer) (11-29-2018)

• Applications: Western Blot, Immunofluorescence,

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