Anticorps Monoclonal anti-MYC-tag
MYC-tag Monoclonal Antibody for IF, IP, WB, ELISA
Hôte / Isotype
Mouse / IgG1
Réactivité testée
Protéine recombinante et plus (3)
Applications
WB, IP, IF, CoIP, ELISA
Conjugaison
Non conjugué
CloneNo.
2D11A8
N° de cat : 66004-1-Ig
Synonymes
"MYC-tag Antibodies" Comparison
View side-by-side comparison of MYC-tag antibodies from other vendors to find the one that best suits your research needs.
Applications testées
| Résultats positifs en WB | Protéine recombinante |
| Résultats positifs en IP | cellules HEK-293 transfectées |
| Résultats positifs en IF | cellules HEK-293 transfectées |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:20000 |
| Immunoprécipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| WB | See 28 publications below |
| IF | See 3 publications below |
| IP | See 7 publications below |
| CoIP | See 4 publications below |
Informations sur le produit
66004-1-Ig cible MYC-tag dans les applications de WB, IP, IF, CoIP, ELISA et montre une réactivité avec des échantillons Protéine recombinante
| Réactivité | Protéine recombinante |
| Réactivité citée | rat, Humain, souris |
| Hôte / Isotype | Mouse / IgG1 |
| Clonalité | Monoclonal |
| Type | Anticorps |
| Immunogène | Peptide |
| Nom complet | MYC-tag |
| Symbole du gène | |
| Identification du gène (NCBI) | |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par protéine A |
| Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The Myc tag corresponds to amino acid residues(EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino- or carboxy- terminus of targeted proteins.
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for MYC-tag antibody 66004-1-Ig | Download protocol |
| IF protocol for MYC-tag antibody 66004-1-Ig | Download protocol |
| IP protocol for MYC-tag antibody 66004-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell PTPS Facilitates Compartmentalized LTBP1 S-Nitrosylation and Promotes Tumor Growth under Hypoxia. | ||
Circ Res Yes-Associated Protein Promotes Angiogenesis Via Signal Transducer and Activator of Transcription 3 in Endothelial Cells. | ||
Nat Commun The ubiquitin ligase UBR5 suppresses proteostasis collapse in pluripotent stem cells from Huntington's disease patients. | ||
Cell Res ERK kinase phosphorylates and destabilizes the tumor suppressor FBW7 in pancreatic cancer. |
