Anticorps Polyclonal de lapin anti-MTUS1

• Phare
• Validé par KD/KO

MTUS1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 13436-1-AP

Synonymes

AT2 receptor binding protein, ATBP, ATIP, GK1, KIAA1288, MP44, MTSG1, MTUS1

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Galerie de données de validation

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  WB analysis using 13436-1-AP

  Jurkat lysates were subjected to SDS PAGE followed by western blot with 13436-1-AP (MTUS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 13436-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13436-1-AP (MTUS1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 13436-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13436-1-AP (MTUS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13436-1-AP

  IP result of anti-MTUS1 (IP:13436-1-AP, 4ug; Detection:13436-1-AP 1:500) with mouse brain tissue lysate 6000ug.

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  IHC staining of human breast cancer using 13436-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13436-1-AP (MTUS1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 13436-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 13436-1-AP (MTUS1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 13436-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 13436-1-AP (MTUS1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 13436-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13436-1-AP (MTUS1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of PC-3 using 13436-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 13436-1-AP (MTUS1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules K-562, tissu cérébral de souris, tissu cérébral humain
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer de la thyroïde humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

13436-1-AP cible MTUS1 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MTUS1 Protéine recombinante Ag4242
• Nom complet: mitochondrial tumor suppressor 1
• Masse moléculaire calculée: 51 kDa, 141 kDa
• Poids moléculaire observé: 40-48 kDa, 80 kDa, 110-120 kDa, 270-280 kDa
• Numéro d’acquisition GenBank: BC033842
• Symbole du gène: MTUS1
• Identification du gène (NCBI): 57509
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MTUS1, also named as ATBP, ATIP, GK1, KIAA1288 and MTSG1, Belongs to the MTUS1 family. It cooperates with AGTR2 to inhibit ERK2 activation and cell proliferation. MTUS1 may be required for AGTR2 cell surface expression. Together with PTPN6, induces UBE2V2 expression upon angiotensin-II stimulation. Isoform 1 inhibits breast cancer cell proliferation, delays the progression of mitosis by prolonging metaphase and reduces tumor growth. MTUS1 up-regulation during cellular transition from proliferation to quiescence and differentiation. It is a potential tumor suppressor gene located at chromosome 8p21.3.22, near marker D8S254. According to the functional data and intracellular localization, MTUS1 also named as mitochondrial tumor suppressor gene 1 (MTSG1). One main feature common to all ATIP members is the presence of a large C-terminal coiled-coil domain that allows homo- and hetero-dimerization of these proteins. (PMID:12692079, 15123706). The antibody can recognize all the isoforms expect isoform 5(85-90 kDa). The antibody tested HomoDimer isoforms (80kd/110-120/280kd) in Jurkat cell.

Publications

Species	Application	Title
mouse	WB	Aging Cell; Activation of angiotensin-converting enzyme 2/angiotensin (1-7)/mas receptor axis triggers autophagy and suppresses microglia proinflammatory polarization via forkhead box class O1 signaling; Authors - Ruili Dang
human	WB,IF,CoIP	FASEB J; Microtubule associated tumor suppressor 1 interacts with mitofusins to regulate mitochondrial morphology in endothelial cells.; Authors - Yinfang Wang
human	WB,IF	J Mol Cell Cardiol; MTUS1 silencing promotes E-selectin production through p38 MAPK-dependent CREB ubiquitination in endothelial cells.; Authors - Yinfang Wang ; KD Validated
mouse	WB	Int J Med Sci; Light Emitting Diodes Irradiation Regulates miRNA-877-3p to Promote Cardiomyocyte Proliferation.; Authors - Xinlu Gao