Anticorps Polyclonal de lapin anti-LCA5

LCA5 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 19333-1-AP

Synonymes

C6orf152, LCA5, Leber congenital amaurosis 5, Lebercilin

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Galerie de données de validation

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  WB analysis of HeLa using 19333-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19333-1-AP (LCA5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 6 publications below
• CoIP: See 1 publications below

Informations sur le produit

19333-1-AP cible LCA5 dans les applications de WB, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LCA5 Protéine recombinante Ag5140
• Nom complet: Leber congenital amaurosis 5
• Masse moléculaire calculée: 693 aa, 80 kDa
• Poids moléculaire observé: 80 kDa
• Numéro d’acquisition GenBank: BC050327
• Symbole du gène: LCA5
• Identification du gène (NCBI): 167691
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LCA5 (Leber congenital amaurosis 5), also named as C6orf152 or Lebercilin, is a 697 amino acids protein. LCA5 is widely expressed at the microtubules, centrosome, and primary cilia. Recent study showed that LCA5 was involved in the cause of congenital and early-onset retinal dystrophies (PMID: 24144451). The MW of this protein is 80 kDa, and this antibody specially recognises the 80 kDa protein.

Publications

Species	Application	Title
mouse		Mol Ther; Amelioration of Neurosensory Structure and Function in Animal and Cellular Models of a Congenital Blindness.; Authors - Ji Yun Song
mouse	IF	Hum Mol Genet; Disruption of the Retinitis Pigmentosa 28 gene Fam161a in mice affects photoreceptor ciliary structure and leads to progressive retinal degeneration.; Authors - Marcus Karlstetter
mouse	IF	Invest Ophthalmol Vis Sci; Treatment Potential for LCA5-Associated Leber Congenital Amaurosis.; Authors - Katherine E Uyhazi
pig	IF	J Proteomics; Tissue- and isoform-specific protein complex analysis with natively processed bait proteins.; Authors - Tina Beyer
human	WB,IF,CoIP	Sci Rep; The interaction between LC8 and LCA5 reveals a novel oligomerization function of LC8 in the ciliary-centrosome system; Authors - Tamás Szaniszló
human	IF	Nat Commun; iU-ExM: nanoscopy of organelles and tissues with iterative ultrastructure expansion microscopy; Authors - Vincent Louvel