Anticorps Polyclonal de lapin anti-ISCU

• Phare
• Validé par KD/KO

ISCU Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

22

Avis (1)

N° de cat : 14812-1-AP

Synonymes

NIFU, ISU2, ISCU2, Iron-sulfur cluster assembly enzyme ISCU, hnifU

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Galerie de données de validation

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  WB analysis of mouse heart using 14812-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14812-1-AP (ISCU antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 14812-1-AP

  WB result of ISCU antibody (14812-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ISCU transfected A549 cells.

• 

  WB analysis of HeLa using 14812-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14812-1-AP (ISCU antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 14812-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 14812-1-AP (ISCU antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 14812-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14812-1-AP (ISCU antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 14812-1-AP

  IP result of anti-ISCU (IP:14812-1-AP, 3ug; Detection:14812-1-AP 1:500) with mouse heart tissue lysate 4000ug.

• 

  IHC staining of human endometrial cancer using 14812-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 14812-1-AP (ISCU Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human endometrial cancer using 14812-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 14812-1-AP (ISCU Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 14812-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14812-1-AP (ISCU Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human lung cancer using 14812-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14812-1-AP (ISCU Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of MCF-7 using 14812-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ISCU antibody (14812-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, cellules A549, cellules COLO 320, cellules HeLa, cellules Raji
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IHC: tissu de cancer de l'endomètre humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 7 publications below
• WB: See 22 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

14812-1-AP cible ISCU dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ISCU Protéine recombinante Ag6562
• Nom complet: iron-sulfur cluster scaffold homolog (E. coli)
• Masse moléculaire calculée: 18 kDa
• Poids moléculaire observé: 14-15 kDa, 18-19 kDa
• Numéro d’acquisition GenBank: BC061903
• Symbole du gène: ISCU
• Identification du gène (NCBI): 23479
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Iron-sulfur (Fe-S) clusters are required for the functions of mitochondrial aconitase, mammalian iron regulatory protein 1, and many other proteins in multiple subcellular compartments. Mitochondrial and cytosolic isoforms of the human Fe-S cluster scaffold protein, iron-sulfur cluster assembly enzyme (ISCU), is involved in activation of iron uptake, redistribution of intracellular iron, and decreased utilization of iron in Fe-S proteins.
Defects in ISCU gene are the cause of myopathy with exercise intolerance Swedish type (MEIS), which is characterized by lifelong severe exercise intolerance (PMID: 18304497). Two ISCU isoforms ISCU1 (cytosolic, 15 kDa) and ISCU2 (mitochondrial, 18 kDa), were produced by alternative splicing, and ISCU2 is considered the canonical isoform1. ISCU2 protein is rapidly processed upon maturation (PMID: 11060020). Proteintech's ISCU antibody 14812-1-AP is raised against the full-length ISCU2 and is expected to detect 14 kDa,15 kDa and 18-19 kDa bands which has been reported in the previous observation (PMID: 11060020).

Publications

Species	Application	Title
human	WB	Science; MMS19 assembles iron-sulfur proteins required for DNA metabolism and genomic integrity.; Authors - Oliver Stehling ; KD Validated
mouse	WB	Cell Res; NDUFAB1 confers cardio-protection by enhancing mitochondrial bioenergetics through coordination of respiratory complex and supercomplex assembly.; Authors - Tingting Hou
human	WB	Cell Metab; Cochaperone binding to LYR motifs confers specificity of iron sulfur cluster delivery.; Authors - Nunziata Maio
mouse	WB	Sci Adv; Macrophage miR-210 induction and metabolic reprogramming in response to pathogen interaction boost life-threatening inflammation.; Authors - Federico Virga
human	WB	Transl Res; Skeletal Muscle MiR-210 Expression is Associated with Mitochondrial Function in Peripheral Artery Disease Patients.; Authors - Ahmed Ismaeel
mouse	WB	Redox Biol; Iron accumulation in senescent cells is coupled with impaired ferritinophagy and inhibition of ferroptosis.; Authors - Shashank Masaldan

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Lana (Verified Customer) (09-06-2020)

SDS-PAGE: 20 ug/ul RIPA protein lysate, 4-12% Bis-Tris gradient gel.Transfer: Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4C.Blocking: SEA Block Blocking Buffer 1h, room T.Primary Ab: O/N incubation at 4C, 1:1000.Secondary Ab: IRDye 800CW Goat anti-Rabbit, 1:15000.Lines of WB image: 1 – protein ladder, 2 - whole cell lysate, 3 – mitochondrial fraction lysate.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse embryonic stem cells

ISCU Antibody Western Blot validation (1:1000 dilution) in Mouse embryonic stem cells (Cat no:14812-1-AP)

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