Anticorps Polyclonal de lapin anti-IL-4

IL-4 Polyclonal Antibody for ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 16545-1-AP

Synonymes

B cell stimulatory factor 1, Binetrakin, BSF 1, BSF1, IL 4, IL4, IL-4, interleukin 4, Pitrakinra

---

Galerie de données de validation

• 

---

Applications testées

Applications publiées

• WB: See 2 publications below
• IF: See 1 publications below
• ELISA: See 1 publications below

Informations sur le produit

16545-1-AP cible IL-4 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IL-4 Protéine recombinante Ag9871
• Nom complet: interleukin 4
• Masse moléculaire calculée: 153 aa, 17 kDa
• Numéro d’acquisition GenBank: BC070123
• Symbole du gène: IL-4
• Identification du gène (NCBI): 3565
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

What is the molecular weight of IL-4?

IL-4 has a calculated weight of 14.9 kDa.

What cell type produces the IL-4 protein?

Mast cells in peripheral tissues produce the cytokines IL-4 and IL-13, both of which regulate response to environmental allergens. IL-4 in particular is synthesized rapidly following T cell activation to then stimulate downstream anti-inflammatory processes.

What activity does IL-4 stimulate?

IL-4 is a highly polyfunctional cytokine, contributing to the regulation of many cellular and immunological processes. Increases in IL-4 levels stimulate IL-13 production as well as helper T cell and B cell proliferative responses to anti-immunoglobulin antibodies. IL-4 also aids in controlling T helper cell type 2 (Th2) differentiation, driving differentiation of naive T cells to the Th2 pathway.

How do IL-4 levels respond to brain damage?

Ischemic neurons may secrete IL-4 to help decrease proinflammatory activity of macrophages.(PMID: 26269636)

How do IL-4 levels respond to allergens?

Asthmatics express stimulated IL-4 bronchoalveolar lavage during allergen challenges.

Can polymorphisms in the Il4 gene lead to asthma?

Polymorphisms resulting in mutant IL-4 proteins, are associated with asthma risk, development, and severity.

What does the IL-4: IFN-g ratio signify?

IL-4 promotes Th2 differentiation and stability, promoting its own production while inhibiting Th1 cell differentiation. The Th1 pathway, however, produces interferon gamma (IFN-g). The IL-4:IFN-g ratio gives indication as to which pathway is being enhanced or depressed.

PMIDs: 26088754, 26255210, 25819429,10556256

Publications

Species	Application	Title
rat	ELISA	Exp Neurol; Photobiomodulation therapy promotes neurogenesis by improving post-stroke local microenvironment and stimulating neuroprogenitor cells.; Authors - Luodan Yang
human	WB	J Transl Med; Immunotherapy landscape analyses of necroptosis characteristics for breast cancer patients.; Authors - Honghao Yu
human	WB	J Hematol Oncol; Ovatodiolide suppresses colon tumorigenesis and prevents polarization of M2 tumor-associated macrophages through YAP oncogenic pathways.; Authors - Yan-Jiun Huang
human	IF	Int J Mol Sci; The Human Cytomegalovirus β2.7 Long Non-Coding RNA Prevents Induction of Reactive Oxygen Species to Maintain Viral Gene Silencing during Latency; Authors - Marianne R Perera
		Surgery; Sodium-glucose co-transporter 2 inhibitor canagliflozin modulates myocardial metabolism and inflammation in a swine model for chronic myocardial ischemia; Authors - Dwight D Harris