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Anticorps Monoclonal anti-IL-1R2

IL-1R2 Monoclonal Antibody for IF, WB,ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

CloneNo.

3H4H4

N° de cat : 60262-1-Ig

Synonymes

CD121b, CDw121b, IL 1R 2, IL 1R beta, IL 1RT 2, IL 1RT2, IL1 Receptor 2, IL-1 Receptor 2, IL1 Receptor II, IL-1 Receptor II, IL1R2, IL-1R2, IL1RB, IL-1RII, Interleukin 1 receptor beta, Interleukin 1 receptor type 2, Interleukin 1 receptor type II

Galerie de données de validation

Filter:
  • Western Blot (WB) analysis of HeLa cells using IL-1R2 Monoclonal antibody (60262-1-Ig)

    WB analysis of HeLa using 60262-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 60262-1-Ig (IL1R2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using IL-1R2 Monoclonal antibody (60262-1-Ig)

    IF Staining of HeLa using 60262-1-Ig

    Immunofluorescent analysis of HeLa cells using 60262-1-Ig (IL1R2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

Applications testées

Résultats positifs en WBcellules HeLa
Résultats positifs en IFcellules HeLa

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

60262-1-Ig cible IL-1R2 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Réactivité citéerat, souris
Hôte / Isotype Mouse / IgG2a
Clonalité Monoclonal
Type Anticorps
Immunogène IL-1R2 Protéine recombinante Ag4495
Nom complet interleukin 1 receptor, type II
Masse moléculaire calculée 398 aa, 45 kDa
Poids moléculaire observé 45 kDa
Numéro d’acquisition GenBankBC039031
Symbole du gène IL1R2
Identification du gène (NCBI) 7850
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Interleukin-1 receptor type 2 (IL1R2, also known as CD121b) is a cytokine receptor that belongs to the interleukin 1 receptor family. It exists in both a membrane bound and soluble form. IL1R2 binds interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA), and acts as a decoy receptor that inhibits the activity of its ligands. IL1R2 serves as a negative regulator of IL-1 signaling, thereby suppressing inflammatory response.

Protocole

Product Specific Protocols
WB protocol for IL-1R2 antibody 60262-1-IgDownload protocol
IF protocol for IL-1R2 antibody 60262-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Mater Today Bio

Intracellular hydrogelation of macrophage conjugated probiotics for hitchhiking delivery and combined treatment of colitis

Authors - Jingzhe Wang
View Article
mouseIF,WB

Biochem Pharmacol

Oridonin employs interleukin 1 receptor type 2 to treat noise-induced hearing loss by blocking inner ear inflammation

Authors - Meng-Bing Chen
View Article