Anticorps Polyclonal de lapin anti-IL-13RA2

• Phare
• Validé par KD/KO

IL-13RA2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 11059-1-AP

Synonymes

CD213A2, CT19, IL 13 receptor subunit alpha 2, IL 13R, IL 13R alpha 2, IL 13R subunit alpha 2, IL 13RA2, IL13BP, IL13R, IL13RA2, IL-13RA2, IL13Ralpha2, Interleukin 13 binding protein

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Galerie de données de validation

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  WB analysis using 11059-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11059-1-AP (IL-13RA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of rat thymus using 11059-1-AP

  rat thymus tissue were subjected to SDS PAGE followed by western blot with 11059-1-AP (IL13RA2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 11059-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 11059-1-AP (IL13RA2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11059-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11059-1-AP (IL13RA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 11059-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11059-1-AP (IL13RA2 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 11059-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11059-1-AP (IL13RA2 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human ovary tumor using 11059-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11059-1-AP (IL13RA2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 11059-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 11059-1-AP (IL13RA2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 11059-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 11059-1-AP (IL13RA2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 11059-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11059-1-AP (IL13RA2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 11059-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11059-1-AP (IL13RA2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 11059-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11059-1-AP (IL-13RA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11059-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11059-1-AP (IL-13RA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HeLa, tissu de thymus de rat, tissu hépatique de rat, tissu testiculaire de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu cérébral humain, tissu de cancer du foie humain, tissu de gliome humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 4 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Informations sur le produit

11059-1-AP cible IL-13RA2 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IL-13RA2 Protéine recombinante Ag1534
• Nom complet: interleukin 13 receptor, alpha 2
• Masse moléculaire calculée: 380 aa, 44 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC020739
• Symbole du gène: IL13RA2
• Identification du gène (NCBI): 3598
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IL13RA2, also named as IL13R and CD213a2, belongs to the type I cytokine receptor family. It binds as a monomer with high affinity to interleukin-13 (IL13), but not to interleukin-4 (IL4). IL13RA2 lacks the cytoplasmic domain for signaling, indicating that it acts as a decoy receptor. IL13RA2 gene polymorphisms have been associated with systemic sclerosis (PMID: 16981293). Overexpression of IL13RA2 gene has been reported in several types of cancer, including melanoma, lung cancer, and brain tumor, which makes it a potential immunotherapeutic target (PMID: 19895199; 24970476; 24021875).

Publications

Species	Application	Title
human	IHC	Neuro Oncol; Correction to: Trivalent CAR T cells overcome interpatient antigenic variability in glioblastoma; Authors -
rat	IF	J Control Release; In situ targeting nanoparticles-hydrogel hybrid system for combined chemo-immunotherapy of glioma.; Authors - Xiaoqi Wang
human	WB,IF,IP	Cancer Biol Med; IL13Rα1 prevents a castration resistant phenotype of prostate cancer by targeting hexokinase 2 for ubiquitin-mediated degradation.; Authors - Tingting Feng ; KD Validated
human	IF	Front Neurosci; Outer brain barriers in rat and human development.; Authors - Christian B Brøchner
human	WB	Front Pharmacol; Isorhamnetin Enhances the Radiosensitivity of A549 Cells Through Interleukin-13 and the NF-κB Signaling Pathway.; Authors - Yarong Du
human,mouse	WB,IHC	Front Physiol; Downregulation of Interleukin-13 Receptor α2 Inhibits Angiogenic Formation Mediated by Chitinase 3-Like 1 in Late Atherosclerotic Lesions of apoE-/- Mice.; Authors - Qi Xue ; KD Validated

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