Anticorps Polyclonal de lapin anti-IGFBP7

• Phare
• Validé par KD/KO

IGFBP7 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, ELISA, IF

Conjugaison

Non conjugué

Publications

8

N° de cat : 19961-1-AP

Synonymes

FSTL2, IBP 7, IGF binding protein 7, IGFBP 7, IGFBP 7v, IGFBP rP1, IGFBP7, IGFBPRP1, MAC25, MAC25 protein, PGI2 stimulating factor, PSF, TAF, Tumor derived adhesion factor

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Galerie de données de validation

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  WB analysis using 19961-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19961-1-AP (IGFBP7 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 19961-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19961-1-AP (IGFBP7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 19961-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19961-1-AP (IGFBP7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse appendix using 19961-1-AP

  IP result of anti-IGFBP7 (IP:19961-1-AP, 4ug; Detection:19961-1-AP 1:300) with mouse appendix tissue lysate 6000ug.

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  IHC staining of human liver using 19961-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19961-1-AP (IGFBP7 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 19961-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19961-1-AP (IGFBP7 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 19961-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19961-1-AP (IGFBP7 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 19961-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19961-1-AP (IGFBP7 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 19961-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19961-1-AP (IGFBP7 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 19961-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19961-1-AP (IGFBP7 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 19961-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19961-1-AP (IGFBP7 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 19961-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19961-1-AP (IGFBP7 Antibody) at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules MOLT-4, cellules SKOV-3, cellules THP-1
• Résultats positifs en IP: tissu d'appendice de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

19961-1-AP cible IGFBP7 dans les applications de WB, IHC, IP, ELISA, IF et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: insulin-like growth factor binding protein 7
• Masse moléculaire calculée: 29 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: NM_001553
• Symbole du gène: IGFBP7
• Identification du gène (NCBI): 3490
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IGFBP7, also named as MAC25, PSF, IBP-7 and TAF, binds IGF-I and IGF-II with a relatively low affinity. IGFBP7 stimulates prostacyclin (PGI2) production. IGFBP7 stimulates cell adhesion.

Publications

Species	Application	Title
mouse	IHC	J Am Soc Nephrol; Mechanisms Underlying Increased TIMP2 and IGFBP7 Urinary Excretion in Experimental AKI.; Authors - Ali C M Johnson
human	IHC,WB	Cancer Lett; IGFBP7-AS1 is a p53-responsive long noncoding RNA downregulated by Epstein-Barr virus that contributes to viral tumorigenesis.; Authors - Wei Dang
mouse	IF	Hum Genet; MS/MS in silico subtraction-based proteomic profiling as an approach to facilitate disease gene discovery: application to lens development and cataract.; Authors - Sandeep Aryal
human	WB	Oral Dis; FERMT2 upregulation in CAFs enhances EMT of OSCC and M2 macrophage polarization; Authors - Xiangrui Ma
human	WB	Heliyon; IGFBP7 promotes gastric cancer by facilitating epithelial-mesenchymal transition of gastric cells; Authors - Jinqing Wang
rat	IHC	J Cell Biochem; IGFBP7 contributes to epithelial-mesenchymal transition of HPAEpiC cells in response to radiation; Authors - Yazhen Zhong