Anticorps Polyclonal de lapin anti-HMGA2

• Phare
• Validé par KD/KO

HMGA2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

37

Avis (3)

N° de cat : 20795-1-AP

Synonymes

BABL, high mobility group AT hook 2, HMGA2, HMGI C, HMGIC, LIPO, STQTL9

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Galerie de données de validation

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  WB analysis using 20795-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20795-1-AP (HMGA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 20795-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20795-1-AP (HMGA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 20795-1-AP

  IP result of anti-HMGA2 (IP:20795-1-AP, 4ug; Detection:20795-1-AP 1:1000) with NIH/3T3 cells lysate 3200ug.

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  IHC staining of human pancreas cancer using 20795-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 20795-1-AP (HMGA2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 20795-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 20795-1-AP (HMGA2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 20795-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20795-1-AP (HMGA2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 20795-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20795-1-AP (HMGA2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 20795-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using HMGA2 antibody (20795-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules C6, cellules HCT 116, cellules HepG2, cellules NCI-H1299, cellules NIH/3T3
• Résultats positifs en IP: cellules NIH/3T3
• Résultats positifs en IHC: tissu de cancer du pancréas humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 30 publications below
• IHC: See 6 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 3 publications below

Informations sur le produit

20795-1-AP cible HMGA2 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HMGA2 Protéine recombinante Ag14588
• Nom complet: high mobility group AT-hook 2
• Masse moléculaire calculée: 108 aa, 12 kDa
• Poids moléculaire observé: 18-20 kDa
• Numéro d’acquisition GenBank: NM_003483
• Symbole du gène: HMGA2
• Identification du gène (NCBI): 8091
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HMGA2 belongs to the family of high mobility group with AT-hook DNA binding domain. HMGA proteins are considered architectural transcription factors; they do not have direct transcriptional activation capacity, but instead regulate gene expression by changing DNA conformation through binding to AT-rich regions in the DNA and/or direct interaction with other transcription factors (PMID: 18202751,19551524). HMGA2 is abundantly and ubiquitously expressed and plays a crucial role during embryonic development (18425117). HMGA2 promotes stem cell self-renewal and research studies have shown that decreased HMGA2 expression is associated with stem cell aging (19551524). Investigators have shown that expression levels of HMGA2 are very low in normal adult tissues, while either overexpression or rearrangement is associated with many types of cancer (PMID: 20228781). The calcualted molecular weight of HMGA2 is 12 kDa, but modified HMGA2 is about 18-20 kDa. (PMID: 18505920 )

Publications

Species	Application	Title
human	WB	Nat Commun; ICAM1 initiates CTC cluster formation and trans-endothelial migration in lung metastasis of breast cancer.; Authors - Rokana Taftaf
human	WB	Nat Commun; N6-methyladenosine modification of circNSUN2 facilitates cytoplasmic export and stabilizes HMGA2 to promote colorectal liver metastasis.; Authors - Ri-Xin Chen
human	WB	Cancer Res; Pooled CRISPR Screening Identifies P-Bodies as Repressors of Cancer Epithelial-Mesenchymal Transition; Authors - Liang Fang
mouse, rat	WB	ACS Appl Mater Interfaces; Layered Double Hydroxide Nanoparticles with Osteogenic Effects as miRNA Carriers to Synergistically Promote Osteogenesis of MSCs.; Authors - Li Yang
human	WB	Cancer Res; YTHDF1 promotes gastric carcinogenesis by controlling translation of FZD7.; Authors - Jingnan Pi
human	CoIP	J Hematol Oncol; MiR-509-3 augments the synthetic lethality of PARPi by regulating HR repair in PDX model of HGSOC.; Authors - Chenggong Sun

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Balawant (Verified Customer) (07-25-2022)

this is very excellent antibody, I have used and it is working great. I will recumbent this antibody if you want for immunoblotting.

• Applications: Western Blot
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: Colon tissue and renal cell line

FH

Susmita (Verified Customer) (04-13-2022)

This antibody is working fine for my WB

• Applications: Western Blot
• Primary Antibody Dilution: WB: 1: 1500
• Cell Tissue Type: CRC cell line

FH

Iram (Verified Customer) (09-17-2020)

HMGA2 bands are very clear and sharp bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000 for western
• Cell Tissue Type: Human colon cancer

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