Anticorps Polyclonal de lapin anti-HLA-F

• Phare
• Validé par KD/KO

HLA-F Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 14670-1-AP

Synonymes

CDA12, HLA 5.4, HLA CDA12, HLA F, HLA F antigen, HLAF, HLA-F, Leukocyte antigen F, MHC class I antigen F

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Galerie de données de validation

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  WB analysis of Raji using 14670-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14670-1-AP (HLA-F antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 14670-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 14670-1-AP (HLA-F antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 14670-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 14670-1-AP (HLA-F antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 14670-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14670-1-AP (HLA-F antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 14670-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14670-1-AP (HLA-F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 14670-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14670-1-AP (HLA-F antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 14670-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14670-1-AP (HLA-F antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 14670-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen slide using 14670-1-AP (HLA-F Antibody) at dilution of 1:50.

• 

  IHC staining of human spleen using 14670-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen slide using 14670-1-AP (HLA-F Antibody) at dilution of 1:50.

• 

  IHC staining of human placenta using 14670-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta slide using 14670-1-AP (HLA-F Antibody) at dilution of 1:50.

• 

  IHC staining of human placenta using 14670-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta slide using 14670-1-AP (HLA-F Antibody) at dilution of 1:50.

• 

  IHC staining of human skin cancer using 14670-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer using 14670-1-AP (HLA-F antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin cancer using 14670-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer using 14670-1-AP (HLA-F antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules Raji, cellules A431, tissu placentaire humain, tissu splénique humain
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu de cancer de la peau humain, tissu placentaire humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 7 publications below
• IF: See 1 publications below

Informations sur le produit

14670-1-AP cible HLA-F dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HLA-F Protéine recombinante Ag6335
• Nom complet: major histocompatibility complex, class I, F
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 40-45 kDa
• Numéro d’acquisition GenBank: BC062991
• Symbole du gène: HLA-F
• Identification du gène (NCBI): 3134
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I and class II. This class I molecules are membrane glycoproteins composed of a heavy (alpha) chain which is encoded by a HLA class I gene, and β2-microglobulin light (beta) chain. The most extensively characterized members of the HLA class I gene family are the genes encoding the major transplantation antigenes, HLA-A, B and C. HLA-F is a non-classical MHC class I molecule. (PMID: 667938; 3375250; 2249951)

Publications

Species	Application	Title
rat	IHC	Kidney Int; Upregulation of HLA-F expression by BK polyomavirus infection induces immune recognition by KIR3DS1-positive natural killer cells.; Authors - Tobias F Koyro
human	IHC	Int J Cancer; Alteration of HLA-F and HLA I antigen expression in the tumor is associated with survival in patients with esophageal squamous cell carcinoma.; Authors - Zhang X X
		J Immunol; Immune Modifications in Fetal Membranes Overlying the Cervix Precede Parturition in Humans.; Authors - Louis Marcellin
mouse,human	WB	Int J Biol Sci; Targeting HLA-F suppresses the proliferation of glioma cells via a reduction in hexokinase 2-dependent glycolysis.; Authors - Xin Chen ; KD Validated
human	WB,IHC	Lung Cancer; HLA-F expression is a prognostic factor in patients with non-small-cell lung cancer.; Authors - Lin Aifen A
human	IHC	Oncol Lett; Lesion human leukocyte antigen-F expression is associated with a poor prognosis in patients with hepatocellular carcinoma.; Authors - Yongfu Xu

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