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Anticorps Polyclonal de lapin anti-HLA class I (HLA-C)

HLA class I (HLA-C) Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

N° de cat : 15777-1-AP

Synonymes

D6S204, HLA C, HLA class I (HLA-C), HLA Cw, HLA Cw12, HLA JY3, HLAC, HLA-C, HLC C, MHC class I antigen Cw*7, PSORS1

Galerie de données de validation

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  • Western Blot (WB) analysis of various lysates using HLA class I (HLA-C) Polyclonal antibody (15777-1-AP)

    WB analysis using 15777-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15777-1-AP (HLA class I (HLA-C) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using HLA class I (HLA-C) Polyclonal antibody (15777-1-AP)

    IP experiment of Jurkat using 15777-1-AP

    IP result of anti-HLA class I (HLA-C) (IP:15777-1-AP, 4ug; Detection:15777-1-AP 1:1000) with Jurkat cells lysate 2400ug.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using HLA class I (HLA-C) Polyclonal antibody (15777-1-AP)

    IHC staining of human lymphoma using 15777-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 15777-1-AP (HLA class I (HLA-C) antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using HLA class I (HLA-C) Polyclonal antibody (15777-1-AP)

    IHC staining of human lymphoma using 15777-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 15777-1-AP (HLA class I (HLA-C) antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HLA class I (HLA-C) Polyclonal antibody (15777-1-AP)

    IF Staining of HeLa using 15777-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15777-1-AP (HLA class I (HLA-C) antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Applications testées

Résultats positifs en WBcellules HL-60, cellules HeLa, cellules HepG2, cellules Jurkat
Résultats positifs en IPcellules Jurkat
Résultats positifs en IHCtissu de lymphome humain
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IF/ICCcellules HeLa

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochimie (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

15777-1-AP cible HLA class I (HLA-C) dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Réactivité citéeHumain
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène HLA class I (HLA-C) Protéine recombinante Ag8461
Nom complet major histocompatibility complex, class I, C
Masse moléculaire calculée 366 aa, 41 kDa
Poids moléculaire observé 44 kDa
Numéro d’acquisition GenBankBC007814
Symbole du gène HLA class I (HLA-C)
Identification du gène (NCBI) 3107
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. This polyclonal antibody raised against human HLA-C can also react with HLA-A and HLA-B. (PMID: 667938; 3375250)

Protocole

Product Specific Protocols
WB protocol for HLA class I (HLA-C) antibody 15777-1-APDownload protocol
IHC protocol for HLA class I (HLA-C) antibody 15777-1-APDownload protocol
IF protocol for HLA class I (HLA-C) antibody 15777-1-APDownload protocol
IP protocol for HLA class I (HLA-C) antibody 15777-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Matern Fetal Neonatal Med

HLA-C promotes proliferation and cell cycle progression in trophoblast cells

Authors - Hong Lv
View Article
humanIHC

Front Pharmacol

Proteomics study the potential targets for Rifampicin-resistant spinal tuberculosis

Authors - Yanling Wang
View Article