Anticorps Polyclonal de lapin anti-GSTP1

• Phare
• Validé par KD/KO

GSTP1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

33

Avis (1)

N° de cat : 15902-1-AP

Synonymes

DFN7, FAEES3, Glutathione S transferase P, glutathione S transferase pi 1, GST class pi, GST3, GSTP1, GSTP1 1, PI

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Galerie de données de validation

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  WB analysis using 15902-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15902-1-AP (GSTP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15902-1-AP

  WB result of GSTP1 antibody (15902-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GSTP1 transfected HeLa cells.

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  WB analysis of human brain using 15902-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15902-1-AP (GSTP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 15902-1-AP

  IP result of anti-GSTP1 (IP:15902-1-AP, 3ug; Detection:15902-1-AP 1:800) with mouse brain tissue lysate 4000ug.

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  IHC staining of human skin using 15902-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15902-1-AP (GSTP1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of mouse skin using 15902-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 15902-1-AP (GSTP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 15902-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 15902-1-AP (GSTP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 15902-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15902-1-AP (GSTP1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 15902-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15902-1-AP (GSTP1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 15902-1-AP

  Immunofluorescent analysis of HepG2 cells using 15902-1-AP (GSTP1 Antibody) at dilution of 1:50 and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of HepG2 using 15902-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug GSTP1 Polyclonal antibody (15902-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules HEK-293, cellules HeLa, cellules Jurkat, cellules K-562, cellules PC-3, tissu cardiaque de rat, tissu cardiaque de souris, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu cutané de souris, tissu cutané humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2
• Résultats positifs en FC (Intra): cellules HepG2
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 25 publications below
• IHC: See 6 publications below
• IF: See 6 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

15902-1-AP cible GSTP1 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GSTP1 Protéine recombinante Ag8731
• Nom complet: glutathione S-transferase pi 1
• Masse moléculaire calculée: 210 aa, 23 kDa
• Poids moléculaire observé: 23-28 kDa
• Numéro d’acquisition GenBank: BC010915
• Symbole du gène: GSTP1
• Identification du gène (NCBI): 2950
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Glutathione S-transferase P (GSTP1) conjugates reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. It is involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2) (PubMed:9084911). Participates in the formation of novel hepoxilin regioisomers (PubMed:21046276).

Publications

Species	Application	Title
human	WB,IP	Mol Cell; SMURF2 predisposes cancer cell toward ferroptosis in GPX4-independent manners by promoting GSTP1 degradation; Authors - Wenxin Zhang ; KD Validated
mouse	WB	Redox Biol; GSTP1-mediated S-glutathionylation of Pik3r1 is a redox hub that inhibits osteoclastogenesis through regulating autophagic flux; Authors - Xiaoxiao Ji ; KD Validated
mouse	IF	J Cell Biol; TDP-43 mediates SREBF2-regulated gene expression required for oligodendrocyte myelination.; Authors - Wan Yun Ho
mouse	WB	Part Fibre Toxicol; Protein target identification and toxicological mechanism investigation of silver nanoparticles-induced hepatotoxicity by integrating proteomic and metallomic strategies.; Authors - Ming Xu
mouse	WB	Cell Death Dis; Poly(ADP-ribosyl)ated PXR is a critical regulator of acetaminophen-induced hepatotoxicity.; Authors - Cheng Wang
human	IHC	Oncotarget; Prognostic significance of GSTP1 in patients with triple negative breast cancer.; Authors - Guanglei Chen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

James (Verified Customer) (11-30-2022)

Works pretty well.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Liver

GSTP1 Antibody Western Blot validation (1/1000 dilution) in Liver (Cat no:15902-1-AP)

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