Anticorps Polyclonal de lapin anti-GSNOR,ADH5

• Phare
• Validé par KD/KO

GSNOR,ADH5 Polyclonal Antibody for WB, IP, IF, IHC, ELISA, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (3)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

40

N° de cat : 11051-1-AP

Synonymes

ADH 3, ADH5, ADHX, Alcohol dehydrogenase 5, Alcohol dehydrogenase class 3, FALDH, FDH, GSH FDH, GSNOR, GSNOR,ADH5

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Galerie de données de validation

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  WB analysis using 11051-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11051-1-AP (GSNOR,ADH5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11051-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11051-1-AP (GSNOR,ADH5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 11051-1-AP

  IP result of anti-GSNOR,ADH5 (IP:11051-1-AP, 4ug; Detection:11051-1-AP 1:5000) with mouse liver tissue lysate 1840 ug.

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  IHC staining of human prostate cancer using 11051-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11051-1-AP (GSNOR,ADH5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 11051-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11051-1-AP (GSNOR,ADH5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 11051-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 11051-1-AP (GSNOR,ADH5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of K-562 using 11051-1-AP

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human GSNOR,ADH5 (11051-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, tissu hépatique de rat, tissu rénal de souris
• Résultats positifs en IP: tissu hépatique de souris,
• Résultats positifs en IHC: tissu de cancer de la prostate humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2
• Résultats positifs en FC (Intra): cellules K-562
• Résultats positifs en cytométrie: cellules K-562

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 9 publications below
• WB: See 32 publications below
• IHC: See 11 publications below
• IF: See 6 publications below
• IP: See 1 publications below

Informations sur le produit

11051-1-AP cible GSNOR,ADH5 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, porc, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GSNOR,ADH5 Protéine recombinante Ag1533
• Nom complet: alcohol dehydrogenase 5 (class III), chi polypeptide
• Masse moléculaire calculée: 40 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: BC070491
• Symbole du gène: ADH5
• Identification du gène (NCBI): 128
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ADH5, also named as ADHX, FDH FALDH GSH-FDH ADH3 and GSNOR, belongs to the zinc-containing alcohol dehydrogenase family and Class-III subfamily. It is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione. ADH5 mediates multiple cardiovascular functions. It plays in regulating heterocellular communication in the artery wall. (PMID:21071693). ADH5 immunostaining is distributed in both the nucleus and cytoplasm of the retinal ganglion cells(PMID:22117533).

Publications

Species	Application	Title
human	WB	Blood; Analysis of disease model iPSCs derived from patients with a novel Fanconi anemia-like IBMFS ADH5/ALDH2 deficiency.; Authors - Anfeng Mu ; KO Validated
human	WB	Nat Commun; Inducing multiple nicks promotes interhomolog homologous recombination to correct heterozygous mutations in somatic cells; Authors - Akiko Tomita
human,mouse	WB,IHC	Mol Cell; Two Aldehyde Clearance Systems Are Essential to Prevent Lethal Formaldehyde Accumulation in Mice and Humans.; Authors - Felix A Dingler ; KO Validated
mouse	WB	Cancer Res; S-nitrosylation of the Mitochondrial Chaperone TRAP1 Sensitizes Hepatocellular Carcinoma Cells to Inhibitors of Succinate Dehydrogenase.; Authors - Salvatore Rizza ; KO Validated
mouse	WB	Antioxid Redox Signal; GSNOR deficiency enhances in situ skeletal muscle strength, fatigue resistance and RyR1 S-nitrosylation without impacting mitochondrial content and activity.; Authors - Younghye Moon ; KO Validated
mouse	WB	Redox Biol; GSNOR modulates hyperhomocysteinemia-induced T cell activation and atherosclerosis by switching Akt S-nitrosylation to phosphorylation.; Authors - Jing Li ; KD Validated

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