Anticorps Polyclonal de lapin anti-Gelsolin

• Phare
• Validé par KD/KO

Gelsolin Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

11

N° de cat : 11644-2-AP

Synonymes

Actin depolymerizing factor, ADF, AGEL, Brevin, Gelsolin, GSN

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Galerie de données de validation

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  WB analysis using 11644-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11644-2-AP (Gelsolin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of U2OS using 11644-2-AP

  U2OS (WT and GSN KO) lysates prepared with RIPA buffer, 25 μg protein loaded. 11644-2-AP incubated at 1:5000 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  IP experiment of U2OS using 11644-2-AP

  U2OS lysates prepared and IP of GSN performed using 1.0 μg of 11644-2-AP coupled to protein A- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  IHC staining of human colon cancer using 11644-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11644-2-AP (Gelsolin Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon cancer using 11644-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11644-2-AP (Gelsolin Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human prostate cancer using 11644-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11644-2-AP (GSN antibody) at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules U2OS
• Résultats positifs en IP: cellules U2OS,
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Informations sur le produit

11644-2-AP cible Gelsolin dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Gelsolin Protéine recombinante Ag2239
• Nom complet: gelsolin (amyloidosis, Finnish type)
• Masse moléculaire calculée: 782 aa, 86 kDa
• Poids moléculaire observé: 87-93 kDa
• Numéro d’acquisition GenBank: BC026033
• Symbole du gène: Gelsolin
• Identification du gène (NCBI): 2934
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Gelsolin (GSN) is an actin binding protein that regulates actin-severing/depolymerizing in a calcium dependent manner, and acts as a multifunctional regulator for cell metabolism and survival. Gelsolin exists in two isoforms: a cytoplasmic gelsolin (cGSN; 80-90 kDa) and a plasma gelsolin (pGSN; 93 kDa), differing at N terminal residues. Decreased levels of pGSN are strongly associated with human diseases, such as, acute liver injury, major trauma, myocardial infarction, inflammation, lung injury, rheumatoid arthritis, hemodialysis, multiple sclerosis, Alzheimer's disease. Plasma gelsolin can interact with actin and form a complex of 130 kDa (PMID: 6330060, 3030756).

Publications

Species	Application	Title
human	WB	Adv Healthc Mater; Modification of PLGA Scaffold by MSC-Derived Extracellular Matrix Combats Macrophage Inflammation to Initiate Bone Regeneration via TGF-β-Induced Protein.; Authors - Moyuan Deng
	WB	Genes Dev; Localization of Kif1c mRNA to cell protrusions dictates binding partner specificity of the encoded protein; Authors - Megan L Norris
human	WB,IF	Am J Cancer Res; 3'untranslated regions (3'UTR) of Gelsolin mRNA displays anticancer effects in non-small cell lung cancer (NSCLC) cells.; Authors - Xiaohui Liu
human	WB, IHC	Gene; Role of gelsolin in cell proliferation and invasion of human hepatocellular carcinoma cells.; Authors - Biao Deng
mouse	WB	Biomed Pharmacother; RACK1 promotes the proliferation, migration and invasion capacity of mouse hepatocellular carcinoma cell line in vitro probably by PI3K/Rac1 signaling pathway.; Authors - Wu Jun J
human	WB	Oncotarget; Correlation of five secretory proteins with the nasopharyngeal carcinoma metastasis and the clinical applications.; Authors - Ya Tao