Anticorps Polyclonal de lapin anti-NMDAR2B/GRIN2B

• Phare
• Validé par KD/KO

NMDAR2B/GRIN2B Polyclonal Antibody for WB, IP, IF, IHC, ELISA, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF-P, FC (Intra), IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

89

N° de cat : 21920-1-AP

Synonymes

GluN2B, GRIN2B, hNR3, NMDAR2B, NMDAR2B/GRIN2B, NR2B, NR3

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Galerie de données de validation

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  WB analysis using 21920-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21920-1-AP (NMDAR2B/GRIN2B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 21920-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21920-1-AP (NMDAR2B/GRIN2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 21920-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21920-1-AP (NMDAR2B/GRIN2B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 21920-1-AP

  IP result of anti-NMDAR2B/GRIN2B (IP:21920-1-AP, 3ug; Detection:21920-1-AP 1:2000) with mouse brain tissue lysate 6000ug.

• 

  IHC staining of mouse brain using 21920-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21920-1-AP (NMDAR2B/GRIN2B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21920-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21920-1-AP (NMDAR2B/GRIN2B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 21920-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21920-1-AP (NMDAR2B/GRIN2B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 21920-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21920-1-AP (NMDAR2B/GRIN2B antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of mouse brain using 21920-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NMDAR2B/GRIN2B antibody (21920-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of SH-SY5Y using 21920-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug NMDAR2B/GRIN2B antibody (21920-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat, tissu cérébral humain
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de souris, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris,
• Résultats positifs en FC (Intra): cellules SH-SY5Y
• Résultats positifs en cytométrie: cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 84 publications below
• IHC: See 8 publications below
• IF: See 11 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below

Informations sur le produit

21920-1-AP cible NMDAR2B/GRIN2B dans les applications de WB, IHC, IF-P, FC (Intra), IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris, pinson zébré
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NMDAR2B/GRIN2B Protéine recombinante Ag16318
• Nom complet: glutamate receptor, ionotropic, N-methyl D-aspartate 2B
• Masse moléculaire calculée: 1484 aa, 166 kDa
• Poids moléculaire observé: 166 kDa
• Numéro d’acquisition GenBank: BC113620
• Symbole du gène: GRIN2B
• Identification du gène (NCBI): 2904
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GRIN2B (also known as GluN2B or NMDAR2B) is a member of the N-methyl-D-aspartate (NMDA) receptor family within the ionotropic glutamate receptor superfamily. NMDA receptors are widely expressed in the central nervous system and play a major role in excitatory synaptic transmission and plasticity (PMID: 23223336). NMDA receptors large multi-subunit complexes arranged into heteromeric assemblies composed of four homologous subunits within a repertoire of over 10 different subunits: eight GluN1 isoforms, four GluN2 subunits (A-D) and two GluN3 subunits (A and B) (PMID: 21395862). Naturally occurring mutations within GRIN2B gene are associated with neurodevelopmental disorders including autism spectrum disorder, attention deficit hyperactivity disorder, epilepsy, and schizophrenia.

Publications

Species	Application	Title
mouse	WB	Adv Sci (Weinh); Single-Nucleus RNA Sequencing Reveals that Decorin Expression in the Amygdala Regulates Perineuronal Nets Expression and Fear Conditioning Response after Traumatic Brain Injury.; Authors - Yingwu Shi
mouse	WB	Sci Adv; Loss of schizophrenia-related miR-501-3p in mice impairs sociability and memory by enhancing mGluR5-mediated glutamatergic transmission; Authors - Wenquan Liang
mouse	WB	Sci Adv; RNF220 is an E3 ubiquitin ligase for AMPA receptors to regulate synaptic transmission; Authors - Pengcheng Ma
human	WB,CoIP	J Clin Invest; TMEM25 modulates neuronal excitability and NMDA receptor subunit NR2B degradation.; Authors - Haiqing Zhang
mouse	WB	Mol Neurodegener; Nuclear speckle specific hnRNP D-like prevents age- and AD-related cognitive decline by modulating RNA splicing.; Authors - Qingyang Zhang
mouse	WB,IF,IP	Cell Mol Life Sci; STIM2 regulates NMDA receptor endocytosis that is induced by short-term NMDA receptor overactivation in cortical neurons; Authors - Karolina Serwach

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