Anticorps Polyclonal de lapin anti-GNRHR

GNRHR Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (2)

Applications

WB, IHC, FC (Intra), ELISA, IF

Conjugaison

Non conjugué

Publications

25

Avis (1)

N° de cat : 19950-1-AP

Synonymes

LHRHR, GRHR, GNRHR1, GnRH-R, GnRH receptor

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Galerie de données de validation

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  WB analysis using 19950-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19950-1-AP (GNRHR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse ovary using 19950-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 19950-1-AP (GNRHR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 19950-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 19950-1-AP (GNRHR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 19950-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19950-1-AP (GNRHR antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 19950-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19950-1-AP (GNRHR antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 19950-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 19950-1-AP (GNRHR Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human ovary using 19950-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 19950-1-AP (GNRHR Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse testis using 19950-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19950-1-AP (GNRHR Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse testis using 19950-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19950-1-AP (GNRHR Antibody) at dilution of 1:200 (under 40x lens).

• 

  FC experiment of MCF-7 using 19950-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human GNRHR (19950-1-AP) (red) or 0.2 ug Control Antibody (blue), and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, tissu ovarien de souris
• Résultats positifs en IHC: tissu ovarien de souris, tissu ovarien humain, tissu testiculaire de souris, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 15 publications below
• IHC: See 9 publications below
• IF: See 7 publications below
• FC: See 2 publications below

Informations sur le produit

19950-1-AP cible GNRHR dans les applications de WB, IHC, FC (Intra), ELISA, IF et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: GnRH receptor
• Masse moléculaire calculée: 38 kDa
• Poids moléculaire observé: 60-70 kDa
• Numéro d’acquisition GenBank: NM_000406
• Symbole du gène: GNRHR
• Identification du gène (NCBI): 2798
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GNRHR, also named GRHR, belongs to the G-protein coupled receptor 1 family. GNRHR is a receptor for GnRH that mediates the action of GnRH to stimulate the secretion of the gonadotropic hormones luteinizing hormone (LH) and follicle-stimulating hormone (FSH). It mediates its action by association with G-proteins that activate a phosphatidylinositol-calcium second messenger system. Isoform2 of GNRHR may act as an inhibitor of GnRH-R signaling. Defects in GNRHR are a cause of idiopathic hypogonadotropic hypogonadism (IHH). Defects in GNRHR are a cause of fertile eunuch syndrome. The antibody only recognizes the isoform1 of GNRHR. The predicted unmodified molecular weight of the human GNRHR is ~38 kDa, the larger band (50-65 kDa) is likely to represent a glycosylated form of GNRHR.

Publications

Species	Application	Title
		Cancer Res; Peptide-Drug Conjugate GnRH-Sunitinib Targets Angiogenesis Selectively at the Site of Action to Inhibit Tumor Growth.; Authors - Orestis Argyros
mouse	WB,IF	Int J Nanomedicine; Pseudoephedrine Nanoparticles Alleviate Adriamycin-Induced Reproductive Toxicity Through the GnRhR Signaling Pathway.; Authors - Yang Fu
human	WB	Int J Mol Sci; Development and Biochemical Characterization of Self-Immolative Linker Containing GnRH-III-Drug Conjugates.; Authors - Sabine Schuster
human	WB	Bioconjug Chem; GnRH-Gemcitabine Conjugates for the Treatment of Androgen-Independent Prostate Cancer: Pharmacokinetic Enhancements Combined with Targeted Drug Delivery.; Authors - Theodoros Karampelas
human	WB	Front Endocrinol (Lausanne); The Anti-proliferative Activity of GnRH Through Downregulation of the Akt/ERK Pathways in Pancreatic Cancer.; Authors - Linna Suo
human	IHC	Endocr Relat Cancer; GnRH antagonist treatment of malignant adrenocortical tumors.; Authors - Milena Doroszko

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Dequina (Verified Customer) (12-11-2018)

This antibody has worked very well for flow cytometry in primary pituitary cells. The positive staining is clear although not bright enough for clean separation of populations even with a very bright secondary (BV421).

• Applications: Flow Cytometry,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Primary Pituitary

GNRHR Antibody Flow Cytometry, validation (1:200 dilution) in Primary Pituitary (Cat no:19950-1-AP)

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