Anticorps Polyclonal de lapin anti-GATA3

• Phare
• Validé par KD/KO

GATA3 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ChIP, ELISA

Conjugaison

Non conjugué

Publications

31

N° de cat : 10417-1-AP

Synonymes

Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3, GATA binding factor 3

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Galerie de données de validation

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  WB analysis using 10417-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10417-1-AP (GATA3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10417-1-AP

  T-47D cells were subjected to SDS PAGE followed by western blot with 10417-1-AP (GATA3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules T-47D

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 21 publications below
• ChIP: See 1 publications below

Informations sur le produit

10417-1-AP cible GATA3 dans les applications de WB, ChIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GATA3 Protéine recombinante Ag0174
• Nom complet: GATA binding protein 3
• Masse moléculaire calculée: 443 aa, 48 kDa
• Poids moléculaire observé: 37 kDa and 48 kDa
• Numéro d’acquisition GenBank: BC003070
• Symbole du gène: GATA3
• Identification du gène (NCBI): 2625
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GATA3 is a transcriptional factor that binds to the enhancer elements of all 4 T-cell antigen receptor genes. GATA3 is highly expressed in naive, freshly activated cells and Th2 lineage cells and thought to be necessary and sufficient for Th2 cytokine gene expression. GATA3 and TBET interfer with each other to regulate gene expression. This is a rabbit polyclonal antibody raised against part of C-terminus of human GATA3.

Publications

Species	Application	Title
human		Cancer Cell; Lunatic fringe deficiency cooperates with the Met/Caveolin gene amplicon to induce basal-like breast cancer.; Authors - Keli Xu
		Cancer Res; Cooperation between Pik3ca and p53 mutations in mouse mammary tumor formation.; Authors - Adams Jessica R JR
	WB	Oxid Med Cell Longev; Androgen Plays a Potential Novel Hormonal Therapeutic Role in Th17 Cells Predominant Neutrophilic Severe Asthma by Attenuating BECs Regulated Th17 Cells Differentiation via MBD2 Expression; Authors - Binaya Wasti
human	WB	Cell Death Dis; Calreticulin promotes EGF-induced EMT in pancreatic cancer cells via Integrin/EGFR-ERK/MAPK signaling pathway.; Authors - Weiwei Sheng
human		Development; The inhibitor of histone acetyltransferases Nir regulates epidermis development.; Authors - Delphine Duteil
	WB	Int Immunopharmacol; Modulating Th1/Th2 drift in asthma-related immune inflammation by enhancing bone mesenchymal stem cell homing through targeted inhibition of the Notch1/Jagged1 signaling pathway; Authors - Wang Kun

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