Anticorps Monoclonal anti-GAPDH

GAPDH Monoclonal Antibody for WB

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, plante, poisson-zèbre, rat, souris et plus (6)

Applications

WB, IP, IHC, IF

Conjugaison

HRP

Publications

552

CloneNo.

1E6D9

Avis (5)

N° de cat : HRP-60004

Synonymes

G3PD, GAPD, GAPDH, HRP-G3PD, HRP-GAPD, HRP-GAPDH, HRP-OK/SW cl.12, OK/SW cl.12

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Galerie de données de validation

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  WB analysis of multi-cells/tissue using HRP-60004

  Western blot analysis of GAPDH in various tissues and cell lines using Proteintech antibody HRP-60004 at a dilution of 1:10000.

• 

  WB analysis of HeLa using HRP-60004

  HeLa cells were subjected to SDS PAGE followed by western blot with HRP-60004 (GAPDH antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using HRP-60004

  HeLa cells were subjected to SDS PAGE followed by western blot with HRP-60004 (GAPDH antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 532 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

HRP-60004 cible GAPDH dans les applications de WB, IP, IHC, IF et montre une réactivité avec des échantillons Humain, plante, poisson-zèbre, rat, souris

• Réactivité: Humain, plante, poisson-zèbre, rat, souris
• Réactivité citée: rat, Humain, Lapin, porc, poulet, souris, Hamster, abeilles, opossum
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: glyceraldehyde-3-phosphate dehydrogenase
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC004109
• Symbole du gène: GAPDH
• Identification du gène (NCBI): 2597
• Conjugaison: HRP
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

Publications

Species	Application	Title
human	WB	Nature; DNA of neutrophil extracellular traps promotes cancer metastasis via CCDC25.; Authors - Linbin Yang
human	WB	Nat Biotechnol; Direct targeting of amplified gene loci for proapoptotic anticancer therapy.; Authors - Meetu Kaushik Tiwari
human	WB	Cell; Programmable RNA-Guided RNA Effector Proteins Built from Human Parts.; Authors - Simone Rauch
human	WB	Cell; Immune Checkpoint Inhibition Overcomes ADCP-Induced Immunosuppression by Macrophages.; Authors - Shicheng Su
human	WB	Cell; CD10+GPR77+Cancer-Associated Fibroblasts Promote Cancer Formation and Chemoresistance by Sustaining Cancer Stemness.; Authors - Shicheng Su
human,mouse	WB	Cell; PMID: 28753426; Authors - Kun Chen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tsimafei (Verified Customer) (07-15-2022)

30µg of total protein were loaded on the gel. Antibodies were incubated ON at 4C.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: mESCs

HRP GAPDH Antibody Western Blot validation (1:5000 dilution) in mESCs (Cat no:HRP-60004)

FH

Hala (Verified Customer) (02-25-2022)

it works very good but i got an aspecific band around 30KDa

• Applications: Western Blot
• Primary Antibody Dilution: 1/15000
• Cell Tissue Type: hepG2

HRP GAPDH Antibody Western Blot validation (1/15000 dilution) in hepG2 (Cat no:HRP-60004)

FH

Laura (Verified Customer) (01-10-2020)

Works well

• Applications: Western Blot,
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: HEK293T

FH

Benjamin (Verified Customer) (01-07-2020)

Works really well with high sensitivity and removes the need for a secondary. Needs to be dilute as GAPDH bands will be too intense at higher dilutions.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:25000
• Cell Tissue Type: HEK293T, SHSY5Y

FH

Kathryn (Verified Customer) (12-10-2019)

Used at 1:5000 in 5% BSA on a small amount of neonatal mouse ovary and worked beautifully.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: mouse ovary

HRP GAPDH Antibody Western Blot, validation (1:5000 dilution) in mouse ovary (Cat no:HRP-60004)

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