Anticorps Polyclonal de lapin anti-GAC-specific

GAC-specific Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

22

Avis (1)

N° de cat : 19958-1-AP

Synonymes

AAD20, GAC, GAC-specific, GLS, GLS1, glutaminase C, K glutaminase, KIAA0838, L glutamine amidohydrolase

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Galerie de données de validation

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  WB analysis using 19958-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19958-1-AP (GAC-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 19958-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19958-1-AP (GAC-specific Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  IP experiment of mouse kidney using 19958-1-AP

  IP result of anti-GAC-specific (IP:19958-1-AP, 3ug; Detection:19958-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

• 

  IHC staining of human heart using 19958-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19958-1-AP (GAC-specific Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human heart using 19958-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19958-1-AP (GAC-specific Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 19958-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 19958-1-AP (GAC-specific Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 19958-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 19958-1-AP (GAC-specific Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human kidney using 19958-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19958-1-AP (GAC-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 19958-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19958-1-AP (GAC-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 19958-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19958-1-AP (GAC-specific Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 19958-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19958-1-AP (GAC-specific Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa
• Résultats positifs en IP: tissu rénal de souris
• Résultats positifs en IHC: tissu cardiaque humain, tissu de cancer du pancréas humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 20 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

19958-1-AP cible GAC-specific dans les applications de WB, IP, IF, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: glutaminase
• Masse moléculaire calculée: 65 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: AF158555
• Symbole du gène: GLS
• Identification du gène (NCBI): 2744
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Glutaminase C (GAC), a splicing variant of the kidney-type glutaminase (KGA) gene, is a vital mitochondrial enzyme protein that catalyzes glutamine to glutamate. GAC is the only isoform present in the mitochondria (PMID:22228304). In a normal state, immunodecorationwith antibodies directed against GAC identified an abundant complex of 232 kDa as a tetrameric form of GAC, the DPAA can directly destructed the native tetrameric GAC complex to accumulate the unassembled one (50-90 kDa). (PMID:22493432). This antibody is specific to GAC isoform. It has no cross reaction to KGA.

Publications

Species	Application	Title
mouse	WB	Cell Metab; Memory CD8+ T Cells Balance Pro- and Anti-inflammatory Activity by Reprogramming Cellular Acetate Handling at Sites of Infection.; Authors - Maria L Balmer
mouse	IF	Cell Stem Cell; Differential Alternative Polyadenylation Landscapes Mediate Hematopoietic Stem Cell Activation and Regulate Glutamine Metabolism.; Authors - Pia Sommerkamp
human	WB,IP	Cancer Commun (Lond); Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer; Authors - Xingbin Hu
human	WB	Blood; Targeting glutaminolysis has anti-leukemic activity in acute myeloid leukemia and synergizes with BCL-2 inhibition.; Authors - Nathalie Jacque
	WB	Elife; Metabolic reprogramming of cancer cells by JMJD6-mediated pre-mRNA splicing associated with therapeutic response to splicing inhibitor; Authors - Carolyn M Jablonowski
human	WB	Oncotarget; Discovery of selective inhibitors of Glutaminase-2, which inhibit mTORC1, activate autophagy and inhibit proliferation in cancer cells.; Authors - Yue-Zhi Lee

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Daniel (Verified Customer) (09-16-2019)

The nitrocellulose membrane was blocked with PBS, 5% BSA, w/ Tween-20.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HK2 cells sustained on glucose or galactose

GAC-specific Antibody Western Blot, validation (1:1000 dilution) in HK2 cells sustained on glucose or galactose (Cat no:19958-1-AP)

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