Anticorps Polyclonal de lapin anti-GABRA4

GABRA4 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 12979-1-AP

Synonymes

GABA A Receptor alpha 4, GABAA Receptor alpha 4, GABRA4

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Galerie de données de validation

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  WB analysis of mouse skeletal muscle using 12979-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12979-1-AP (GABRA4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 12979-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12979-1-AP (GABRA4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 12979-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12979-1-AP (GABRA4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 12979-1-AP

  IP result of anti-GABRA4 (IP:12979-1-AP, 4ug; Detection:12979-1-AP 1:500) with mouse skeletal muscle tissue lysate 2200ug.

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Applications testées

• Résultats positifs en WB: tissu de muscle squelettique de souris, tissu cérébral de souris, tissu rénal de souris, tissu rénal humain
• Résultats positifs en IP: tissu de muscle squelettique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

12979-1-AP cible GABRA4 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GABRA4 Protéine recombinante Ag3600
• Nom complet: gamma-aminobutyric acid (GABA) A receptor, alpha 4
• Masse moléculaire calculée: 554 aa, 62 kDa
• Poids moléculaire observé: 62-70 kDa
• Numéro d’acquisition GenBank: BC035055
• Symbole du gène: GABRA4
• Identification du gène (NCBI): 2557
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Gamma-aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. GABRA4, encoding the extrasynaptically located α4‐subunit of the GABAA receptor, is involved in the etiology of autism. (PMID: 32028044, 32033586)

Publications

Species	Application	Title
mouse	WB	FASEB J; Electro-acupuncture alleviates adolescent cocaine exposure-enhanced anxiety-like behaviors in adult mice by attenuating the activities of PV interneurons in PrL.; Authors - Jiaxun Nie
human	IP,WB	Cell Mol Life Sci; Cyclin D1-Cdk4 regulates neuronal activity through phosphorylation of GABAA receptors; Authors - Neus Pedraza
human	WB	Genes Dis; The Traf2 and NcK interacting kinase inhibitor NCB-0846 suppresses seizure activity involving the decrease of GRIA1; Authors - Min Wang

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