Anticorps Polyclonal de lapin anti-FGFR2
FGFR2 Polyclonal Antibody for IHC, IF/ICC, FC (Intra), ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain et plus (1)
Applications
IHC, IF/ICC, FC (Intra), ELISA
Conjugaison
Non conjugué
N° de cat : 13042-1-AP
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en IHC | tissu de cancer du sein humain, tissu de cancer de la prostate humain il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
| Résultats positifs en IF/ICC | cellules HepG2 |
| Résultats positifs en FC (Intra) | cellules HepG2, |
Dilution recommandée
| Application | Dilution |
|---|---|
| Immunohistochimie (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| IHC | See 1 publications below |
| IF | See 1 publications below |
Informations sur le produit
13042-1-AP cible FGFR2 dans les applications de IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain
| Réactivité | Humain |
| Réactivité citée | Humain, souris |
| Hôte / Isotype | Lapin / IgG |
| Clonalité | Polyclonal |
| Type | Anticorps |
| Immunogène | FGFR2 Protéine recombinante Ag3701 |
| Nom complet | fibroblast growth factor receptor 2 |
| Masse moléculaire calculée | 709 aa, 79 kDa |
| Poids moléculaire observé | |
| Numéro d’acquisition GenBank | BC039243 |
| Symbole du gène | FGFR2 |
| Identification du gène (NCBI) | 2263 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par affinité contre l'antigène |
| Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
FGFR2 (Fibroblast growth factor receptor 2) is a tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration, and apoptosis. Ligand binding leads to the activation of several signaling pathways, such as RAS, MAPK1/ERK2, MAPK3/ERK1, and the MAP Kinase signaling pathway, as well as the AKT1 signaling pathway. Mutations in the gene of FGFR2 are associated with Crouzon syndrome, Pfeiffer syndrome, Craniosynostosis, Apert syndrome, Jackson-Weiss syndrome, Beare-Stevenson cutis gyrata syndrome, Saethre-Chotzen syndrome, and syndromic craniosynostosis. This polyclonal antibody raised against 358-704aa of human FGFR2 can cross-react with other members of the FGFR family.
Protocole
| Product Specific Protocols | |
|---|---|
| IHC protocol for FGFR2 antibody 13042-1-AP | Download protocol |
| IF protocol for FGFR2 antibody 13042-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Am J Cancer Res miR-381-3p suppresses the proliferation of oral squamous cell carcinoma cells by directly targeting FGFR2. | ||
Nephron The Effects of M1/M2 Macrophages on the mRNA Expression Profile of Diabetic Glomerular Endothelial Cells. | ||
Anim Biotechnol MiR-125b regulates the differentiation of hair follicles in Fine-wool Sheep and Cashmere goats by targeting MXD4 and FGFR2. | ||
Life (Basel) MPP7 as a Novel Biomarker of Esophageal Cancer: MPP7 Knockdown Inhibits Esophageal Cancer Cell Migration and Invasion | ||
Tissue Eng Part A The UC-MSCbFGF/scaffold system accelerates the healing of the uterine full-thickness injury |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Kus (Verified Customer) (12-08-2022) | Works good WB but could see working for Immunofluorescence.
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FH K (Verified Customer) (02-02-2022) | This Ab works great for immunofluorescence and IHC at 1:200 dilution but this Ab did not work well for western blotting.
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