Anticorps Monoclonal anti-EPHA1-specific

EPHA1-specific Monoclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

1B7C3

N° de cat : 60155-2-Ig

Synonymes

EPH, EPH receptor A1, EPHA1, Ephrin type A receptor 1, EPHT, EPHT1

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Galerie de données de validation

• 

  WB analysis of HeLa using 60155-2-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60155-2-Ig (EPHA1-specific antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 60155-2-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 60155-2-Ig (EPHA1-specific antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 60155-2-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60155-2-Ig (EPHA1-specific antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 60155-2-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60155-2-Ig (EPHA1-specific antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 60155-2-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60155-2-Ig (EPHA1-specific antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 60155-2-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60155-2-Ig (EPHA1-specific antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 60155-2-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60155-2-Ig (EPHA1-specific antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 60155-2-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60155-2-Ig (EPHA1-specific antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 60155-2-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60155-2-Ig (EPHA1-specific antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules COLO 320, cellules HepG2
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer de l'estomac humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

60155-2-Ig cible EPHA1-specific dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: EPH receptor A1
• Masse moléculaire calculée: 108 kDa
• Poids moléculaire observé: 108 kDa
• Numéro d’acquisition GenBank: BC130291
• Symbole du gène: EPHA1
• Identification du gène (NCBI): 2041
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Précipitation de l'acide caprylique/du sulfate d'ammonium
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EPHA1, also named as EPH, EPHT and EPHT1, belongs to ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. Ligands in the ephrin-A subclass, including the prototype family member ephrin-A1 (B61), are membrane associated through glycosylphosphatidyl-inositol linkages, whereas ephrin-B subclass consists of ligands with transmembrane domains. The general role of the Eph family is in mediating repulsive cell-cell interaction.

Publications

Species	Application	Title
human	WB	Metab Brain Dis; Sequencing and Bioinformatics analysis of lncRNA/circRNA-miRNA-mRNA in Glioblastoma multiforme; Authors - Renjie Wang

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