Anticorps Polyclonal de lapin anti-CRACR2A

• Phare
• Validé par KD/KO

CRACR2A Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 15206-1-AP

Synonymes

CRAC channel regulator 2A, CRACR2A, EFCAB4B, Rab46

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Galerie de données de validation

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  WB analysis of Jurkat using 15206-1-AP

  WB result of CRACR2A antibody (15206-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CRACR2A transfected Jurkat cells.

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  WB analysis of Jurkat using 15206-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15206-1-AP (CRACR2A antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15206-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15206-1-AP (CRACR2A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of Daudi using 15206-1-AP

  Daudi cells were subjected to SDS PAGE followed by western blot with 15206-1-AP (CRACR2A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 15206-1-AP

  IP result of anti-CRACR2A (IP:15206-1-AP, 4ug; Detection:15206-1-AP 1:500) with Jurkat cells lysate 3200ug.

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  IHC staining of human tonsillitis using 15206-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15206-1-AP (CRACR2A antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 15206-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15206-1-AP (CRACR2A antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 15206-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 15206-1-AP (CRACR2A antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules Daudi, cellules HeLa
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu d'amygdalite humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Informations sur le produit

15206-1-AP cible CRACR2A dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CRACR2A Protéine recombinante Ag7358
• Nom complet: EF-hand calcium binding domain 4B
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: 46 kDa, 80-90 kDa
• Numéro d’acquisition GenBank: BC004524
• Symbole du gène: CRACR2A
• Identification du gène (NCBI): 84766
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CRACR2A (also called CRAC regulator 2A, EFCAB4B) is a cytoplasmic Ca2+ sensor predominantly expressed in lymphocytes. It has two isoforms: a long isoform of CRACR2A-a round 80-90 kDa and a short isoform of CRACR2A-c around 46 kDa. CRACR2A-c is involved in the regulation of Ca2+-NFAT signaling. CRACR2A-a exists in a GTP- and Golgi membrane-bound form in resting T cells, while it is present in subsynaptic vesicles that translocated from the Golgi region to the immunological synapse after ligation of TCRs. This antibody recognizes both of CRACR2A-c and CRACR2A-a.

Publications

Species	Application	Title
human	WB,IP	Elife; Biallelic mutations in calcium release activated channel regulator 2A (CRACR2A) cause a primary immunodeficiency disorder.; Authors - Beibei Wu
mouse	WB	J Immunol; CRACR2A-Mediated TCR Signaling Promotes Local Effector Th1 and Th17 Responses.; Authors - Jin Seok Woo
human	IP,IF,WB	Sci Rep; Affinity-based proteomics reveals novel binding partners for Rab46 in endothelial cells.; Authors - Lucia Pedicini
human	WB	Biochem Biophys Res Commun; A long variant of CRACR2A that belongs to the Rab GTPase protein family.; Authors - Lesley A Wilson ; KD Validated
human	WB,IF	J Cell Biol; Rab46 integrates Ca2+ and histamine signaling to regulate selective cargo release from Weibel-Palade bodies.; Authors - Katarina T Miteva ; KD Validated
mouse	IF	Discov Immunol; Rab46: a novel player in mast cell function; Authors - Lucia Pedicini

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